TAKEDA Junji ≪Environmental and Molecular Medicine≫ “Genome-wide phenotype analysis in ES cells by regulated disruption of the Bloom’s syndrome gene.”

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Publish Nature 429, 896-899, 2004

The limitation of phenotype-based genetic screening in mammalian systems is the diploid nature of the genome. We used a tetracycline-regulated Bloom’s syndrome gene to introduce bi-allelic mutations across the genome in mouse ES cells.

URL http://www.casi.osaka-u.ac.jp/25/