TAKEDA Junji ≪Environmental and Molecular Medicine≫ “Region-specific saturated germ-line mutagenesis in mice using the Sleeping Beauty transposon system.”
Publish Nat. Methods.2; 763-769, 2005
We use the ‘local hopping’ phenomenon of a DNA-type transposon, Sleeping Beauty (SB), for region-specific saturation mutagenesis. All genes within a 4-Mb region of the original donor site were mutated by SB, indicating the potential of this system for functional genomic studies within a specific chromosomal region.