TAKEDA Junji ≪Environmental and Molecular Medicine≫ “Region-specific saturated germ-line mutagenesis in mice using the Sleeping Beauty transposon system.”

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Publish Nat. Methods.2; 763-769, 2005

We use the ‘local hopping’ phenomenon of a DNA-type transposon, Sleeping Beauty (SB), for region-specific saturation mutagenesis. All genes within a 4-Mb region of the original donor site were mutated by SB, indicating the potential of this system for functional genomic studies within a specific chromosomal region.

URL http://www.casi.osaka-u.ac.jp/25/