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論文 招待講演 学会発表

論文

2017

Kawai T, Tatsumi S, Kihara S, Sakimura K, Okamura Y. (2017) Mechanistic insight into the suppression of microglial ROS production by voltage-gated proton channels (VSOP/Hv1) Channels (Austin) in press.

Kawai T, Okochi Y, Ozaki T, Imura Y, Koizumi S, Yamazaki M, Abe M, Sakimura K, Yamashita T, Okamura Y (2017) Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production. J. Neurochem. 142(5), 686-699

Ratanayotha A, Kawai T, Higashijima S, Okamura Y (2017) Molecular and functional characterization of the voltage-gated proton channel in zebrafish neutrophils Physiol. Reports 5(15), e133345

Hirai S, Hotta K, Kubo Y, Nishino A, Okabe S, Okamura Y, Okado H (2017) AMPA glutamate receptors are required for sensory-organ formation and morphogenesis in the basal chordate. Proc. Natl. Acad. Sci. U. S. A. 114(15), 3939-3944

Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda M, and Nagai T (2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Scientific Reports 7:42398

2016

岡村 康司 (2016) 電位依存性プロトンチャネルにおけるプロトン透過機構について 生物物理 56(3):154-158

Kasuya G, Hiraizumi M, Maturana AD, Kumazaki K, Fujiwara Y, Liu K, Nakada-Nakura Y, Iwata S, Tsukada K, Komori T, Uemura S, Goto Y, Nakane T, Takemoto M, Kato HE, Yamashita K, Wada M, Ito K, Ishitani R, Hattori M, Nureki O.(2016) Crystal structures of the TRIC trimeric intracellular cation channel orthologues.Cell Research 26:1288-1301

Sakata S, Miyawaki N, McCormack TJ, Arima H, Kawanabe A, Ozkucur N, Kurokawa T, Jinno Y, Fujiwara Y, Okamura Y.(2016) Comparison between mouse and sea urchin orthologs of voltage-gated proton channel suggests role of S3 segment in activation gating. Biochem. Biophys. Acta. 1858(12):2972-2983

Okamura Y. (2016) Simple scheme of lipid enzyme can explain complex lives of phosphoinositides. Proc. Natl. Acad. Sci. U. S. A. 113(26):7012-7014

Sakata S, Jinno Y, Kawanabe A, Okamura Y.(2016) Voltage-dependent motion of the catalytic region of voltage-sensing phosphatase monitored by a fluorescent amino acid. Proc. Natl. Acad. Sci. U. S. A. 113(27):7521-7526

Fujiwara Y, Kondo H, Shirota M, Kobayashi M, Takeshita K, Nakagawa A, Okamura Y, Kinoshita K. (2016) Structural basis for the membrane association of ankyrinG via palmitoylation. Scientific Reports 6:23981

Okuda H, Yonezawa Y, Takano Y, Okamura Y, Fujiwara Y.(2016) Direct Interaction between the Voltage-sensors Produces Cooperative Sustained Deactivation in Voltage-gated H+ Channel Dimers. J. Biol. Chem. 291(11):5935-47

Minato Y, Suzuki S, Hara T, Kofuku Y, Kasuya G, Fujiwara Y, Igarashi S, Suzuki E, Nureki O, Hattori M, Ueda T, Shimada I. (2016) Conductance of P2X4 purinergic receptor is determined by conformational equilibrium in the transmembrane region. Proc. Natl. Acad. Sci. USA 113:4741-4746

Kasuya G, Fujiwara Y, Takemoto M, Dohmae N, Nakura Y, Ishitani R, Hattori M, Nureki O. (2016) Structural Insights into Divalent Cation Modulations of ATP-Gated P2X Receptor Channels. Cell Reports 14:932-944

Kawanabe A, Okamura Y.(2016) Effects of unsaturated fatty acids on the kinetics of voltage-gated proton channels heterologously expressed in cultured cells. J. Physiol. 594(3):595-610.

Okochi Y, Aratani Y, Adissu HA, Miyawaki N, Sasaki M, Suzuki K, Okamura Y.(2016) The voltage-gated proton channel Hv1/VSOP inhibits neutrophil granule release.J. Leukoc. Biol. 99(1):7-19.

2015

Okamura Y, Fujiwara Y, Sakata S.(2015) Gating mechanisms of voltage-gated proton channels. Annual Rev. Biochem.84:685-709.

Berthier C, Kutchukian C, Bouvard C, Okamura Y, Jacquemond V.(2015) Depression of voltage-activated Ca2+ release in skeletal muscle by activation of a voltage-sensing phosphatase. J. Gen. Physiol. 145(4):315-30. doi: 10.1085/jgp.201411309.

Kawai T, Yoshimura A, Oka Y. (2015) Neurones in the preoptic area of the male goldfish are activated by a sex pheromone 17α,20β-dihydroxy-4-pregnen-3-one. J Neuroendocrinol. 27(2):123-30.

2014

Ohtsuka Y, Matsumoto J, Katsuyama Y, Okamura Y. (2014) Nodal signaling regulates specification of ascidian peripheral neurons through control of the BMP signal. Development 141(20):3889-99.

Yamaguchi S, Aoki N, Kitajima T, Okamura Y, Homma KJ. (2014) Expression of the voltage-sensing phosphatase gene in the chick embryonic tissues and in the adult cerebellum. Com. Integ. Biol. 7(5):1-4.

Fujiwara Y, Okamura Y.(2014) Temperature-sensitive gating of voltage-gated proton channels. Current Topics in Membranes 74:259-292

Fujiwara Y, Kurokawa T, Okamura Y.(2014) Long α helices projecting from the membrane as the dimer interface in the voltage-gated H+ channel. J. Gen. Physiol. 143:377-386

Mutua J, Jinno Y, Sakata S, Okochi Y, Ueno S, Tsutsui H, Kawai T, Iwao Y, Okamura Y.(2014) Functional diversity of voltage-sensing phosphatases (VSPs) in two urodele amphibians. Physiological Reports 2 : no. e12061, DOI: 10.14814/phy2.12061

Tsutsui H, Jinno Y, Tomita A, Okamura Y.(2014) Rapid evaluation of a protein-based voltage probe using a field-induced membrane potential change.Biochem Biophys Acta. 1838(7):1730-7. doi: 10.1016/j.bbamem.2014.03.002.

Takeshita K, Sakata S, Yamashita E, Fujiwara Y, Kawanabe A, Kurokawa T, Okochi Y, Matsuda M, Narita H, Okamura Y*, Nakagawa A* (2014) X-ray crystal structure of voltage-gated proton channel. Nature Structural & Molecular Biology Published online: 02 March 2014 | doi:10.1038/nsmb.2783, *equal to correspondence

Itsuki K, Imai Y, Hase H, Okamura Y, Inoue R, Mori M. (2014) PLC-mediated PI(4,5)P2 hydrolysis regulates activation and inactivation of TRPC6/7 channels. J. Gen. Physiol.143(2):183-201.

Kokunai Y, Nakara T, Furuta M, Sakata S, Kimura H, Aiba T, Yoshinaga M, Osaki Y, Nakamori M, Itoh H, Sato T, Kubota T, Kadota K, Shindo K, Mochizuki H, Shimizu W, Horie M, Okamura Y, Ohno K, Takahashi MP. (2014) A Kir3.4 mutation causes Andersen-Tawil syndrome by an inhibitory effect on Kir2.1. Neurology82(12):1058-64.

Sakata S, Okamura Y. (2014) Phosphatase activity of the voltage-sensing phosphatase, VSP, shows graded dependence on the extent of activation of the voltage sensor. J. Physiol. 592(5):899-914

2013

Kurokawa T, Okamura Y. (2013) Mapping of sites facing aqueous environment of voltage-gated proton channel at resting state: A study with PEGylation protection. Biochem. Biophys. Acta. 1838(1):382-7.

Yamaguchi S, Kurokawa T, Taira I, Aoki N, Sakata S, Okamura Y, Homma KJ. (2013) Potential role of voltage-sensing phosphatases in regulation of cell structure through the production of PI(3,4)P2. J. Cell. Physiol. 229(4):422-33.

Tsutsui H, Jinno Y, Tomita A, Niino Y, Yamada Y, Mikoshiba K, Miyawaki A, Okamura Y. (2013) Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase. J. Physiol. 591(18):4427-37.

Tsutsui H, Jinno Y, Tomita A, Okamura Y. (2013) Optically detected structural change in the N-terminal region of the voltage-sensor domain. Biophys. J. 105(1):108-15.

Fujiwara Y, Takeshita K, Nakagawa A, Okamura Y. (2013) Structural characteristics of the redox-sensing coiled coil in the voltage-gated H+ channel. J. Biol. Chem. 288(25):17968-75.

Kawai T, Abe H, Oka Y. (2013) Burst generation mediated by cholinergic input in terminal nerve-gonadotrophin releasing hormone neurones of the goldfish. J Physiol. 591(22):5509-23.

2012

Sasaki M, Tojo A, Okochi Y, Miyawaki N, Kamimura D, Yamaguchi A, Murakami M, Okamura Y. (2012) Autoimmune disorder phenotypes in HVCN1 gene deficient mice. Biochem. J., 450(2):295-301.

Fujiwara Y, Kurokawa T, Takeshita K, Nakagawa A, Larsson HP, Okamura Y. (2012) Gating of the Designed Trimeric/Tetrameric Voltage-Gated H+ Channel. J. Physiol., 591(Pt 3):627-40.

Itsuki K, Imai Y, Okamura Y, Abe K, Inoue R, Mori MX. (2012) Voltage-sensing phosphatase reveals temporal regulation of TRPC3/C6/C7 channels by membrane phosphoinositides. Channels (Austin), 1;6(3)

Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Horie S, Homma KJ, Sasaki T & Okamura Y. (2012). 3’ phosphatase activity toward PI(3,4)P2 by voltage-sensing phosphatase, VSP. Proc. Natl. Acad. Sci. U. S. A., 109(25):10089-94.

Fujiwara Y, Kurokawa T, Takeshita K, Kobayashi M, Okochi Y, Nakagawa A & Okamura Y. (2012). The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H+ channel Hv1.,Nature Communications, 3:816. doi: 10.1038/ncomms1823.

Okamura Y.(2012) Voltage-gated proton channels., In: Edward H. Egelman, editor: Comprehensive Biophysics, Vol 6, Channels Proteins, Montal, M. Oxford: Academic Press, pp.199-222.

Imai Y, Itsuki K, Okamura Y, Inoue R, Mori MX (2012) A self-limiting regulation of TRPC3/C6/C7 channels coupled with PI(4,5)P2-diacylglycerol signalling. The Journal of Physiology,590(Pt 5):1101-19.

2011

Nakajo K, Nishino A, Okamura Y, Kubo Y (2011) KCNQ1 subdomains involved in KCNE modulation revealed by an invertebrate KCNQ1 orthologue. J. Gen. Physiol. 138(5):521-35.

Ratzan WJ, Evsikov AV, Okamura Y, Jaffe LA. (2011) Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence. J. Cell Physiol., 226(11):2740-6.

Matsuda M, Takeshita K, Kurokawa T, Sakata S, Suzuki M, Yamashita E, Okamura Y, Nakagawa A (2011)Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity.The Journal of Biological Chemistry, 286(26):23368-77.

Sakata S, Hossain MI, Okamura Y (2011) Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range of voltage sensitivity. The Journal of Physiology, 589(11):2687-705. Link

Okamura Y & Dixon JE (2011) Voltage-sensing phosphatase: its molecular relationship with PTEN. Physiology, 26(1):6-13.download

Nishino A, Baba SA & Okamura Y (2011) A Mechanism for Graded Motor Control Encoded in the Channel Properties of the Muscle ACh Receptor. Proc. Natl. Acad. Sci. U. S. A., 108(6): 2599-2604. Link

2010

岡村康司 (2010) 膜電位情報をイノシトールリン脂質シグナルへ変換する 膜タンパク質VSP. 実験医学増刊「脂質生物学」, 100-105.

Okamura Y (2010) A global synergy of ions and voltage? J. Physiol. 588(23):4649.

Ogasawara M, Sasaki M, Nakazawa N, Nishino A & Okamura Y (2010), Gene expression profile of Ci-VSP in juveniles and adult blood cells of ascidian.Gene Expression Patterns, in press

Tsutsui H, Higashijima S, Miyawaki A & Okamura Y(2010),Visualizing Voltage Dynamics in Zebrafish Heart.J. Physiol. 588(12):2017-21.

Nishino A, Okamura Y, Piscopo S & Brown ER (2010), A glycine receptor is involved in the organization of swimming movements in an invertebrate chordate. BMC Neurosci. 11:6.

El Chemaly A, Okochi Y, Sasaki M, Arnaudeau S, Okamura Y & Demaurex N (2010), VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification J. Exp. Med. 207(1):129-39.

Sakata S, Kurokawa T, Norholm MH, Takagi M, Okochi Y, von Heijne G & Okamura Y (2010), Functionality of the voltage-gated proton channel truncated in S4 Proc. Natl. Acad. Sci. U. S. A. 107(5):2313-8.

2009

Okamura Y (2009), Another story of arginines in voltage sensing: the role of phosphoinositides in coupling voltage sensing to enzyme activity J. Gen. Physiol. 134(1):1-4.

Okamura Y, Murata Y & Iwasaki H (2009), Voltage-sensing phosphatase: actions and potentials J. Physiol. Topical Review, 587(3) 513-520.

Okochi Y, Sasaki M, Iwasaki H, Okamura Y (2009), Voltage-gated proton channel is expressed on phagosomes Biochem. Biophys. Res. Commun. 382(2):274-9.

2008

Hill AS, Nishino A, Nakajo K, Zhang G, Fineman JR, Selzer ME, Okamura Y & Cooper EC (2008), Ion channel clustering at the axon initial segment and node of Ranvier evolved sequentially in early chordates. PLoS Genetics, (12):e1000317.

Musset, B. , Cherny, V.V., Morgan, D., Okamura, Y, Ramsey, I. S., Clapham, D. E. & *DeCoursey T.E. (2008), Detailed comparison of expressed and native voltage-gated proton channel currents J. Physiol. 586, 2477-86.

Hossain, M.I. , Iwasaki, H., Okochi, Y., Chahine, M., Higashijima, S., Nagayama K. & *Okamura, Y. (2008) Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs J. Biol. Chem. 283, 18248-18259.

Koch, H.P.#, Kurokawa, T.#, Okochi, Y., Sasaki, M., *Okamura, Y & *Larsson, H.P., Multimeric nature of voltage-gated proton channels. Proc. Natl. Acad. Sci. U.S.A., (2008) Jul 1;105(26):9111-6. Epub 2008 Jun 26. (# Equal contribution, * shared corresponding authors)

*Matsumoto,J., Katsuyama, Y., Ohtsuka, Y., Lemaire, P. & Okamura, Y (2008) Functional analysis of synaptotagmin gene regulatory regions in two distantly related ascidian species. Dev. Growth Differ. 50, 543-552.

Iwasaki H, Murata Y, Kim Y, Hossain MI, Worby CA, Dixon JE, McCormack T, Sasaki T & * Okamura, Y (2008). A voltage-sensing phosphatase, Ci-VSP, which shares sequence identity with PTEN, dephosphorylates phosphatidylinositol 4,5-bisphosphate. Proc Natl Acad Sci U S A 105, 7970-7975.

Tsutsui, H., Karasawa, S., Okamura, Y & *Miyawaki, A. (2008). Improving membrane voltage measurements using FRET with new fluorescent proteins. Nature Methods, 8,683-5.

2007

Ohtsuka Y & *Okamura Y. Voltage-dependent calcium influx mediates maturation of myofibril arrangement in ascidian larval muscle. Dev. Biol., 301:361-373. (2007)

Murata Y & *Okamura Y. (2007) Depolarization activates the phosphoinositide phosphatase Ci-VSP, as detected in Xenopus oocytes coexpressing sensors of PIP2. J. Physiol. , 583, 875-889.

*Okamura Y. (2007) Biodiversity of voltage sensor domain proteins. (review) Pflugers Archiv.,454(3):361-71.

2006

Sasaki M, Takagi M & *Okamura Y. A voltage sensor-domain protein is a voltage-gated proton channel, Science, 312(5773), 589-92. Epub 2006 Mar 23.(2006).

Shirahata E, Iwasaki H, Takagi M, Lin C, Bennett V, *Okamura Y & Hayasaka K. Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6. J. Neurophysiol. 96(3):1347-57. (2006)

Kimura Y, Okamura Y & *Higashijima S. “alx, a zebrafish homolog of Chx10, marks ipsilateral descending excitatory interneurons that participate in the regulation of spinal locomotor circuits” J. Neurosci. 26(21), 5684-97. (2006)

2005

Miyamoto T, Morita K, Takemoto D, Takeuchi K, Kitano Y, Miyakawa T, Nakayama K, Okamura Y, Sasaki H, Miyachi Y, Furuse M & *Tsukita S. Tight junctions in Schwann cells of peripheral myelinated axons: a lesson from claudin-19-deficient mice. J. Cell Biol., 169(3):527-38.(2005).

*Katsuyama Y, Okada T, Matsumoto J, Ohtsuka Y, Terashima T & Okamura Y. Early specification of ascidian larval motor neurons. Dev. Biol., 278(2):310-22.(2005).

Murata Y, Iwasaki H, Sasaki M, Inaba K & *Okamura Y. Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor. Nature, 435:1239-1243.(2005).

*Okamura Y, Nishino A, Murata Y, Nakajo K., Iwasaki H, Ohtsuka Y, Tanaka-Kunishima M, Takahashi N, Hara Y, Yoshida T, Nishida M, Okado H, Watari H, Meinertzhagen IA, Satoh N, Takahashi K, Satou Y, Okada Y & Mori M. Comprehensive analysis of the ascidian genome reveals novel insights into the molecular evolution of ion channel genes. Physiological Genomics, 22(3):269-82.(2005).

*Brown ER, Nishino A, Meinertzhagen IA, Bone Q & Okamura Y. GABAergic synaptic transmission modulates swimming in the ascidian larva. Eur. J. Neurosci., 22(10):2541-8. (2005).

*Chahine M, Ziane R,Vijayaragavan K & Okamura Y. Regulation of Nav channels in sensory neurons. (review) Trends Pharmacol. Sci., Oct;26(10):496-502. (2005).

2004

Nakajo K & *Okamura Y. Development of transient outward currents coupled with Ca2+-induced Ca2+ release mediates oscillatory membrane potential in ascidian muscle. J. Neurophysiol., 92(2):1056-66.(2004).

*Meinertzhagen IA, Lemaire Y & Okamura Y. The neurobiology of the ascidian tadpole larva: recent developments in an ancient chordate. (review) Annual Rev. Neurosci., 27:453-485. (2004).

2003

*Nakajo K, Katsuyama Y, Ono F, Ohtsuka Y & Okamura Y. Identification, functional characterization and developmental expression of the ascidian Kv4-class potassium channel. Neurosci. Res., 45:59-70.(2003).

*Izumi-Nakaseko H, Yamaguchi S, Ohtsuka Y, Ebihara T, Adachi-Akahane S & Okamura Y. DHP-insensitive L-type-like Ca channel of ascidian acquires sensitivity to DHP with single amino acid change in domain III P-region. FEBS letters, 549:67-71.(2003).

*Okamura Y, Izumi-Nakaseko H, Nakajo K, Ohtsuka Y & Ebihara T. The ascidian dihydropyridine-resistant calcium channel as the prototype of chordite L-type calcium channel. (review) Neurosignals, 12(3):142-158. (2003).

2002

Katsuyama Y, Matsumoto J, Okada T, Ohtsuka Y, Cheng L, Okado H & Okamura Y. Regulation of synaptotagmin gene expression during ascidian embryogenesis. Dev. Biol., 244(2):293-304.(2002).

Okada T, Katsuyama Y, Ono F & Okamura Y. The development of three identified motor neurons in ascidian embryo. Dev. Biol., 244(2):278-92.(2002).

Ebihara T, Komiya Y, Izumi-Nakaseko H, Adachi-Akahane S, Okabe S & Okamura Y. Coexpression ofa Cav1.2 protein lacking an N-terminus and the first domain specifically suppresses L-type calcium channel activity. FEBS Letters, 529:203,(2002).

2001

Ohtsuka Y, Okamura Y, & Obinata T. Changes in gelsolin expression during ascidian metamorphosis. Development Gene& Evolution, 211; 252-256.(2001).

Okada T, Stanley McIsaac S, Okamura Y & Meinertzhagen, I.A. The form of neurons in the ascidian central nervous system of the tadpole larva of Ciona . Biol. Bull., 200: 252-25,(2001).

Ohtsuka Y, Obinata T & Okamura Y. Induction of ascidian peripheral neurons by vegetal blastomeres. Dev. Biol.239(1):107-117.(2001).

Okagaki R., Izumi H, Okada T, Nagahora H, Nakajo K, & Okamura Y. The maternal transcript for truncated voltage-dependent Ca2+ channel in ascidian embryos; a potential suppressive role for Ca2+ channel expression. Dev. Biol., 230: 258-277.(2001).

Murata Y, Okado H, Katsuyama Y, Okamura Y & Kubo Y. Primary structure, developmental expression and functional properties of an inward rectifier K+ channel of the tunicate. Receptors & Channels, 7: 387-399.(2001).

Meinertzhagen IA & Okamura Y. The larval ascidian nervous system: the chordate brain from its small beginnings. (review) Trends in Neurosci. 24:401-10. (2001).

- 2000

  2000年以前の業績



招待講演

2015

Okamura Y. "How is VSP's enzyme activity activated by intrinsic voltage sensor?" RECI V 5th Spanish Ion Channel Network Meeting, 2015/10/4-6, Barcelona, Spain

岡村康司 "最小イオンチャネルHv1/VSOPによる貪食細胞の活性酸素産生の多重制御" 第24回日本バイオイメージング学会学術集会, 2015/9/28, 東京

Okamura Y. "Determinants of activation rate of voltage-gated proton channel revealed by chimeric constructs between two orthologs" TMMU Neuroscience Symposium : Ion Channels, 2015/6/30-7/1, Chongqing, China

Okamura Y. "Fine tuning of neutrophil activities by the voltage-gated proton channel, Hv1" The 5th International Ion Channel Conference, 2015/6/26-28, Luzhou, China

Okamura Y. "Molecular mechanisms of voltage sensing phosphatase, VSP" 岡崎統合バイオサイエンスセンター サマースクール2015 Development of Biosensing Research, 2015/8/212-13, Okazaki

学会発表

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