{"id":5466,"date":"2019-11-08T13:47:38","date_gmt":"2019-11-08T04:47:38","guid":{"rendered":"http:\/\/www.med.osaka-u.ac.jp\/eng\/?page_id=5466"},"modified":"2022-08-26T14:57:30","modified_gmt":"2022-08-26T05:57:30","slug":"kishikawa-okada201911","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2019year\/kishikawa-okada201911","title":{"rendered":"Toshihiro Kishikawa,Yukinori Okada \u226aStatistical Genetics\u226b <span>When your microbiome and your genome aren\u2019t a good combination<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li><a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2019year\/okada-kishikawa2019-11\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p>2019-11-08<br \/><em><\/p>\n<p><span class=\"lineFrame\">Publish\u00a0<\/span> <em>Annals of the Rheumatic Diseases<\/p>\n<p><em>Researchers led by a team from Osaka University find a link between the gut microbiome, the host genome, and rheumatoid arthritis in Japanese patients<\/em><\/p>\n<p class=\"figure\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-5468 size-medium\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-1-400x194.png?_t=1574916571\" alt=\"\" width=\"400\" height=\"194\" srcset=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-1-400x194.png 400w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-1-768x372.png 768w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-1-1024x496.png 1024w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-1.png 1299w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-1.png\"><span class=\"caption\"><br \/><span class=\"click\">Click to enlarge<\/span><\/span><\/a><\/p>\n<p>One of the most astounding discoveries of recent times is just how much influence gut bacteria have on our health and well-being. In addition to extracting nutrients from food, the collective activity of these tiny organisms protects us from infection and helps regulate our immune system. However, changes in the gut microbiota have been implicated in diseases ranging from obesity and diabetes to inflammatory bowel disease and cancer.<\/p>\n<p>In a study published this month in the <em>Annals of the Rheumatic Diseases<\/em>, researchers led by a team from Osaka University have found that the RA gut metagenome of the Japanese population had interesting characteristics and a population-specific pathway link with the host genome.<\/p>\n<p>Rheumatoid arthritis is a common autoimmune disorder causing painful inflammation and damage to the joints of affected patients. Researchers have so far linked a whole host of genetic and environmental factors to the onset of rheumatoid arthritis, with the microbiota recently emerging as a possible contributing factor. However, while overstimulation of the immune response by specific bacteria has been implicated in the development of rheumatoid arthritis, the way in which the microbiota as a whole contributes to the pathogenesis of rheumatoid arthritis remains the subject of much debate.<\/p>\n<p>\u201cWe carried out a genetic screen using whole-genome shotgun sequencing of the entire gut microbial community in patients with rheumatoid arthritis and compared it to that of healthy controls to get a better idea of which bacterial species, genes, and pathways may contribute to the onset of rheumatoid arthritis in Japanese patients,\u201d explains lead author of the study Toshihiro Kishikawa.<\/p>\n<p>This screen, called a metagenome-wide association study (MWAS), showed an abundance of various bacteria belonging to the genus <em>Prevotella<\/em> in the gut microbiota of rheumatoid arthritis patients, indicating that more species than were previously thought may be involved in disease etiology. In addition, the study identified several genes and biological pathways that were significantly enriched in the rheumatoid arthritis metagenome.<\/p>\n<p>\u201cWe then compared the enriched pathways in the bacterial metagenome with those shown to be enriched in the genomes of East Asian and European patients with rheumatoid arthritis,\u201d says senior author of the study Yukinori Okada. \u201cInterestingly, while several overlapping pathways were identified between the metagenome and the host genomes, the correlation was only significant for the East Asian population, suggesting a population-specific link between the host genome and the metagenome in the pathology of rheumatoid arthritis.\u201d<\/p>\n<p>\u201cWe hope that by establishing this novel link, our work will further the development of treatment strategies to combat rheumatic diseases.\u201d<\/p>\n<p>The article, \u201cMetagenome-wide association study of gut microbiome revealed novel aetiology of rheumatoid arthritis in the Japanese population,\u201d was published in <em>Annals of the Rheumatic Diseases<\/em> at DOI: 10.1136\/annrheumdis-2019-215743.<\/p>\n<p><strong>Summary:<br \/><\/strong>Research carried out by a team led by Osaka University has shown that various <em>Prevotella<\/em> species, along with several specific genes and biological pathways, are enriched in the gut microbiota of Japanese patients with rheumatoid arthritis. When the enriched biological pathways in the gut microbial metagenome were compared with enriched pathways in the host genome, a population-specific link was identified between the gut metagenome, the host genome, and the pathology of rheumatoid arthritis.<\/p>\n<p><strong>Primary Keyword:<\/strong> Biology<br \/><strong>Additional Keywords:<\/strong> Microbiology, Molecular Biology, Genetics, Immunology\/Allergies\/Asthma<\/p>\n<p><strong>Overall process:<br \/><\/strong>We collected fecal samples from 82 patients with rheumatoid arthritis (RA) and 42 healthy controls. We conducted a shotgun sequencing-based metagenome-wide association study, which consisted of three major bioinformatic analytic techniques for case-control comparisons: phylogenetic analysis, functional gene analysis, and pathway analysis. To assess the taxonomic relative abundance data, we conducted non-linear unsupervised clustering analysis and assessed correlations between clustering and the results of the case-control phylogenetic association tests. In addition, we compared pathway enrichment data for the metagenome with that generated by a host genome-wide association study in RA patients (East Asians, n = 22,515; Europeans, n = 58,284).<\/p>\n<p><strong>Title:<\/strong>\u201d Metagenome-wide association study of gut microbiome revealed novel aetiology of rheumatoid arthritis in the Japanese population\u201c<br \/><strong>Journal:<\/strong> Annals of the Rheumatic Diseases<\/p>\n<p><strong>Authors:<\/strong> Toshihiro Kishikawa, Yuichi Maeda, Takuro Nii, Daisuke Motooka, Yuki Matsumoto, Masato Matsushita, Hidetoshi Matsuoka, Maiko Yoshimura, Shoji Kawada, Satoru Teshigawara, Eri Oguro, Yasutaka Okita, Keisuke Kawamoto, Shinji Higa, Toru Hirano, Masashi Narazaki, Atsushi Ogata, Yukihiko Saeki, Shota Nakamura, Hidenori Inohara, Atsushi Kumanogoh, Kiyoshi Takeda, Yukinori Okada<\/p>\n<p>&nbsp;<\/p>\n<p>DOI: 10.1136\/annrheumdis-2019-215743<\/p>\n<p>Funded by: Japan Society for the Promotion of Science, Japan Agency for Medical Research and Development, Takeda Science Foundation, Osaka University Graduate School of Medicine, and Immunology Frontier Research Center (WPI-\u00adIFReC), Osaka University<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-5482\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/IMG_3047-1024x683.jpg?_t=1574917785\" alt=\"\" width=\"600\" height=\"400\" srcset=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/IMG_3047-1024x683.jpg 1024w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/IMG_3047-400x267.jpg 400w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2019\/11\/IMG_3047-768x512.jpg 768w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese 2019-11-08 Publish\u00a0 Annals of the Rheumatic Diseases Researchers led by a team from Osaka Uni [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":5468,"parent":4390,"menu_order":95,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/5466"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=5466"}],"version-history":[{"count":14,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/5466\/revisions"}],"predecessor-version":[{"id":7608,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/5466\/revisions\/7608"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/4390"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/5468"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=5466"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}