{"id":6288,"date":"2020-11-26T09:59:23","date_gmt":"2020-11-26T00:59:23","guid":{"rendered":"http:\/\/www.med.osaka-u.ac.jp\/eng\/?page_id=6288"},"modified":"2022-08-26T14:47:54","modified_gmt":"2022-08-26T05:47:54","slug":"nakamura2020","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2020year\/nakamura2020","title":{"rendered":"Shuhei Nakamura, Satoshi Minami\u3001Saki Shigeyama, Tmotsu Yoshimori \u226aGenetics\u226b <span>Lipids, lysosomes, and autophagy: The keys to preventing kidney injury<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li><a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2020year\/2020-10iden\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><em><\/p>\n<p><span class=\"lineFrame\">Publish\u00a0<\/span> <em>Nature Cell Biology<\/em><strong><br \/><\/strong><\/p>\n<p><em>A team of researchers led by Osaka University unravel a tangle of pathways involving calcium, lipids, and the degradation of damaged lysosomes that is essential for preventing crystal-induced kidney injury<\/em><strong>\u00a0<\/strong><\/p>\n<p class=\"figure\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-6292 size-medium\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-400x209.png?_t=1606354033\" alt=\"\" width=\"400\" height=\"209\" srcset=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-400x209.png 400w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d-768x402.png 768w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d.png 1007w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/4c91d76fc4075d5b09d799ccb1e3fc4d.png\"> <span class=\"caption\"><strong>Figure 1 LC3 lipidation is essential for TFEB activation. TFEB is localized to the nucleus upon lysosomal damage in WT cells, while ATG7 KO cells, in which LC3 lipidation is defective, show impaired TFEB nuclear localization. <strong><\/p>\n<p><span class=\"click\">Click to enlarge<\/span><\/strong><\/strong><\/span><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>Human cells need to work like well-oiled machines to keep our bodies running as they should. Waste products such as misfolded proteins, damaged cellular components, and carbohydrates get in the way and must be quickly disposed of. Dealing with this cellular \u201ctrash\u201d are spherical, membrane-bound organelles called lysosomes filled with a mixture of potent enzymes. In a process called autophagy, waste products are contained within a double-membraned vesicle, called an autophagosome, that fuses with a lysosome. The lysosomal enzymes then get to work breaking down the waste into components that can be recycled.<\/p>\n<p>The problem with lysosomes is that if they are ruptured, their contents can leak out and cause serious damage to the cell. Calcium oxalate crystal-induced kidney injury, which is linked to the progression of chronic kidney disease, is actually the result of lysosomal damage caused by the crystals. It is not surprising then that cells have several pathways to repair or quickly eliminate damaged lysosomes. Yet the exact steps in these pathways and how they interact during the lysosomal damage response are not entirely clear.<\/p>\n<p>In a study published in <em>Nature Cell Biology<\/em>, a team of researchers led by Osaka University have finally unraveled the interactions among the lysosomal damage response pathways and determined how they prevent oxalate-induced kidney injury.<\/p>\n<p>\u201cA protein called TFEB turns on genes necessary for autophagy and the production of new lysosomes in response to lysosomal damage,\u201d explains lead author Shuhei Nakamura. \u201cBy inhibiting TFEB function in HeLa cells and then inducing lysosome damage, we confirmed that TFEB is activated upon lysosomal damage and is necessary for the removal of damaged lysosomes.\u201d<\/p>\n<p>Attachment of lipids to a protein called LC3 is an essential step in the formation of the autophagosome. To their surprise, the researchers also found that lipidated LC3 was necessary for the activation of TFEB during the lysosomal damage response, but there was no clear link between the systems.<\/p>\n<p>\u201cCalcium is a known activator of TFEB,\u201d says senior author Tamotsu Yoshimori. \u201cTo identify how the TFEB and LC3 systems overlapped, we investigated lysosomal calcium channel TRPML1. We found that lipidated LC3 was recruited by lysosomes in response to damage, and that the lipidated protein interacted with TRPML1, causing increased calcium efflux from the lysosome, which activated TFEB.\u201d<\/p>\n<p>The physiological importance of this interaction was then confirmed using a mouse model of oxalate crystal-induced kidney damage. Mice lacking TFEB had more severe kidney damage compared with control animals. Understanding how these pathways interact is the first step in preventing lysosomal damage-associated diseases.<\/p>\n<p>The article, \u201cLC3 lipidation is essential for TFEB activation during the lysosomal damage response upon kidney injury,\u201d was published in <em>Nature Cell Biology<\/em> at DOI: https:\/\/doi.org\/XXX<\/p>\n<p><strong>Summary:<\/strong> Lysosomes are cellular waste disposal organelles containing potent enzymes that cause cellular damage if they leak out of ruptured lysosomes. In a recent study led by Osaka University, researchers found that several distinct pathways involved in the repair or elimination of damaged lysosomes work together in response to lysosomal damage. The proper activation and function of these pathways was essential for preventing kidney injury in a mouse model of oxalate crystal-induced kidney damage.<\/p>\n<p><strong>Primary Keyword:<\/strong> Biology<br \/><strong>Additional Keywords:<\/strong> Cell Biology, Molecular Biology, Physiology, Internal Medicine<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p class=\"figure\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-6296 size-medium\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/f523dc7eb9d216d6d93339cee7e1a627-400x350.jpg?_t=1606354342\" alt=\"\" width=\"400\" height=\"350\" srcset=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/f523dc7eb9d216d6d93339cee7e1a627-400x350.jpg 400w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/f523dc7eb9d216d6d93339cee7e1a627-768x672.jpg 768w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/f523dc7eb9d216d6d93339cee7e1a627-1024x896.jpg 1024w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/f523dc7eb9d216d6d93339cee7e1a627.jpg 1035w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2020\/11\/f523dc7eb9d216d6d93339cee7e1a627.jpg\"> <span class=\"caption\"><strong>Figure 2 Functional TFEB is required to prevent the progression of crystal nephropathy. <br \/><\/strong>In the crystal nephropathy mouse model, TFEB-deficient mice showed increased severity of kidney injury and lysosomal damage compared with control mice. <br \/><strong><br \/><span class=\"click\">Click to enlarge<\/span><\/strong><\/span><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>Title: \u201cLC3 lipidation is essential for TFEB activation during the lysosomal damage response\u00a0upon kidney injury\u201d<br \/>Journal: <em>Nature Cell Biology<br \/><\/em>Authors: Shuhei Nakamura, Saki Shigeyama, Satoshi Minami,\u00a0Takayuki Shima, Shiori Akayama,\u00a0Tomoki Matsuda,\u00a0Alessandra Esposito,\u00a0Gennaro Napolitano,\u00a0Akiko Kuma,\u00a0Tomoko Namba-Hamano, Jun Nakamura,\u00a0Kenichi\u00a0Yamamoto, Miwa Sasai, Ayaka Tokumura, Mika Miyamoto,\u00a0Yukako\u00a0Oe, Toshiharu Fujita,\u00a0Seigo\u00a0Terawaki, Atsushi Takahashi,\u00a0Maho\u00a0Hamasaki, Masahiro\u00a0Yamamoto, Yukinori\u00a0Okada, Masaaki\u00a0Komatsu,\u00a0Takeharu\u00a0Nagai,\u00a0Yoshitsugu\u00a0Takabatake,\u00a0Haoxing\u00a0Xu,\u00a0Yoshitaka Isaka, Andrea\u00a0Ballabio, Tamotsu Yoshimori<\/p>\n<p>Funded by: Japan Science and Technology Agency<\/p>\n<p>&nbsp;<\/p>\n<p><strong>About Osaka University <\/strong><\/p>\n<p>Osaka University was founded in 1931 as one of the seven imperial universities of Japan and is now one of Japan&#8217;s leading comprehensive universities with a broad disciplinary spectrum. This strength is coupled with a singular drive for innovation that extends throughout the scientific process, from fundamental research to the creation of applied technology with positive economic impacts. Its commitment to innovation has been recognized in Japan and around the world, being named Japan&#8217;s most innovative university in 2015 (Reuters 2015 Top 100) and one of the most innovative institutions in the world in 2017 (Innovative Universities and the Nature Index Innovation 2017). Now, Osaka University is leveraging its role as a Designated National University Corporation selected by the Ministry of Education, Culture, Sports, Science and Technology to contribute to innovation for human welfare, sustainable development of society, and social transformation.<\/p>\n<p>Website: <a href=\"https:\/\/resou.osaka-u.ac.jp\/en\/top\">https:\/\/resou.osaka-u.ac.jp\/en\/top<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Publish\u00a0 Nature Cell Biology A team of researchers led by Osaka University unravel a tangle o [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":6296,"parent":5550,"menu_order":61,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/6288"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=6288"}],"version-history":[{"count":16,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/6288\/revisions"}],"predecessor-version":[{"id":7587,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/6288\/revisions\/7587"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/5550"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/6296"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=6288"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}