{"id":651,"date":"2017-03-14T11:50:54","date_gmt":"2017-03-14T02:50:54","guid":{"rendered":"http:\/\/www2.med.osaka-u.ac.jp\/wwwmed\/eng\/activities\/results\/2008year\/tsukita-sachiko"},"modified":"2017-04-28T15:31:06","modified_gmt":"2017-04-28T06:31:06","slug":"article03","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2008year\/article03","title":{"rendered":"TSUKITA Sachiko \u226aBiological Science\u226b <span>\u201cIQGAP3 regulates cell proliferation through the Ras\/ERK signalling cascade\u201d<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2008year\/article03\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><!-- \n\n<h1>\u6708\u7530 \u65e9\u667a\u5b50 \u226a\u5206\u5b50\u751f\u4f53\u60c5\u5831\u5b66\u226b<\/h1>\n\n --><\/p>\n<p class=\"figure\"><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2008\/tukita200808.jpeg\" target=\"_blank\" class=\"zoom\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2008\/tukita200808.jpeg\" alt=\"\" height=\"200\" width=\"85\" \/><br \/>\n  <span class=\"click\">Click to enlarge<\/span><\/a><\/p>\n<p>2008-8<br \/>\n  <span class=\"lineFrame\">Publish<\/span> Nature Cell Biology, 10:971-978, 2008<\/p>\n<p><!-- \n\n<h3>IQGAP3\u306f\u3001Ras\/ERK\u3092\u4ecb\u3057\u3066\u7d30\u80de\u5897\u6b96\u3092\u5236\u5fa1\u3059\u308b\u3002<\/h3>\n\n\n\n\n<p>\u8981\u65e8\uff1a\u751f\u4f53\u306b\u304a\u3044\u3066\u3001\u7d30\u80de\u5897\u6b96\u304c\u3044\u304b\u306b\u6642\u7a7a\u9593\u7684\u306b\u5236\u5fa1\u3055\u308c\u308b\u304b\u306f\u3001\u751f\u4f53\u306e\u6a5f\u80fd\u306e\u307f\u306a\u3089\u305a\u3001\u767a\u751f\u3001\u5206\u5316\u306e\u4e0a\u3067\u3082  \u91cd\u8981\u3067\u3042\u308a\u3001\u7d30\u80de\u63a5\u7740\u3068\u95a2\u308f\u308b\u554f\u984c\u3067\u3042\u308b\u3068\u601d\u308f\u308c\u308b\u3002\u672c\u7814\u7a76\u3067\u306f\u3001\u7d30\u80de\u63a5\u7740\u90e8\u4f4d\u306b\u5c40\u5728\u3059\u308bIQGAP3\u304c\u7d30\u80de\u5897\u6b96\u304c\u884c\u308f\u308c\u3066\u3044\u308b\u7d30\u80de\u306e\u307f\u306b\u767a\u73fe\u3057\u3001\u6d3b\u6027\u5316  \u578bRas\u3068\u5f37\u304f\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002\u3053\u308c\u306b\u3088\u308aIQGAP3\u304cRas\u306e\u6d3b\u6027\u5316\u578b\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u3053\u3068\u304c\u8003\u3048\u3089\u308c\u3001IQGAP3\u306e\u767a\u73fe\u3092\u304a\u3055\u3048\u305f\u30ce\u30c3\u30af\u30c0  \u30a6\u30f3\u7d30\u80de\u306b\u304a\u3044\u3066Ras\u306e\u6d3b\u6027\u5316\u72b6\u6cc1\u3092\u8abf\u3079\u308b\u3068\u3001Ras\u306e\u6d3b\u6027\u306f\u4f4e\u3044\u72b6\u614b\u306b\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u305f\u3002\u307e\u305fIQGAP3\u30ce\u30c3\u30af\u30c0\u30a6\u30f3\u7d30\u80de\u306b\u3001\u6d3b\u6027\u5316\u578bRas\u3092\u767a  \u73fe\u3055\u305b\u308b\u3068\u7d30\u80de\u5897\u6b96\u304c\u76db\u3093\u306b\u306a\u3063\u305f\u3002\u4ee5\u4e0a\u306e\u3053\u3068\u304b\u3089IQGAP3\u306fRas\/ERK\u306e\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u7d4c\u8def\u3092\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u3067\u7d30\u80de\u306e\u5897\u6b96\u3092\u7dad\u6301\u3057\u3066\u304a\u308a\u3001\u305d\u306e\u5c40  \u5728\u304b\u3089\u5897\u6b96\u306e\u63a5\u89e6\u963b\u5bb3\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002\u30de\u30a6\u30b9\u500b\u4f53\u30ec\u30d9\u30eb\u306e\u5c0f\u8178\u306b\u304a\u3044\u3066\u306f\u3001\u5e79\u7d30\u80de\u306b\u7531\u6765\u3059\u308b\u5897\u6b96\u306e\u6d3b\u767a\u306a\u7d30\u80de\u3067\uff29\uff31\uff27\uff21\uff30\uff13\u304c\u50cd\u304f\u3053\u3068\u3082\u78ba\u8a8d  \u3067\u304d\u305f\u3002<br \/>\n  \u3053\u306e\u3088\u3046\u306b\u3001\uff29\uff31\uff27\uff21\uff30\uff13\u306f\u5206\u88c2\u3059\u308b\u7d30\u80de\u3059\u3079\u3066\u3067\u50cd\u3044\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3001\u7279\u306b\u304c\u3093\u907a\u4f1d\u5b50Ras\u306e\u5236\u5fa1\u306b\u95a2\u4fc2\u3057\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u3001\uff29\uff31\uff27\uff21\uff30\uff13\u3092\u3046\u307e\u304f\u5236\u5fa1\u3067  \u304d\u308c\u3070\u764c\u306e\u6cbb\u7642\u306b\u3082\u3064\u306a\u304c\u308b\u3068\u671f\u5f85\u3067\u304d\u308b\u3002\u518d\u751f\u533b\u7642\u306b\u5fdc\u7528\u3059\u308c\u3070\u3001\u79fb\u690d\u306b\u5fc5\u8981\u306a\u7d30\u80de\u3092\u7d20\u65e9\u304f\u5897\u3084\u305b\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u3001\u4eca\u5f8c\u306e\u9032\u5c55\u304c\u671f\u5f85\u3055\u308c\u308b\u3002<\/p>\n\n --><\/p>\n<p>Proliferation of epithelial cells must be   spatiotemporally regulated to maintain the organization of epithelial   sheets. Here we show that the IQGAP family, comprising IQGAP1, 2 and 3,   underlies lateral cell-cell contacts of epithelial cells. Of the three   proteins, IQGAP3 is unique in that its expression is specifically   confined to proliferating cells. Knockdown of <em>IQGAP3<\/em> in cultured   epithelial cells caused inhibition of proliferation and ERK activity.   When exogenously expressed in quiescent cells, IQGAP3 was capable of   inducing cell-cycle re-entry, which was completely inhibited by the MEK   inhibitor U0126. Thus, IQGAP3 is necessary and sufficient for driving   cell proliferation and ERK acts downstream of IQGAP3. Furthermore,   IQGAP3 specifically interacted with the active, GTP-bound form of Ras,   and in <em>IQGAP3<\/em> knockdown cells, the activity of Ras, but not of   other small GTPases, was inhibited. Thus, IQGAP3 regulates the promotion   of cell proliferation through Ras-dependent ERK activation.<\/p>\n<p class=\"mt25\"><span class=\"lineFrame\">URL<\/span> <a href=\"http:\/\/www.fbs.osaka-u.ac.jp\/labs\/tsukita\/\" target=\"_blank\">http:\/\/www.fbs.osaka-u.ac.jp\/labs\/tsukita\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Click to enlarge 2008-8 Publish Nature Cell Biology, 10:971-978, 2008 Proliferation of epithe [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1814,"parent":463,"menu_order":169,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/651"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=651"}],"version-history":[{"count":7,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/651\/revisions"}],"predecessor-version":[{"id":2370,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/651\/revisions\/2370"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/463"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/1814"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=651"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}