{"id":652,"date":"2017-03-14T11:51:31","date_gmt":"2017-03-14T02:51:31","guid":{"rendered":"http:\/\/www2.med.osaka-u.ac.jp\/wwwmed\/eng\/activities\/results\/2009year\/yoneda-yoshihiro"},"modified":"2017-04-28T15:31:25","modified_gmt":"2017-04-28T06:31:25","slug":"article01","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2009year\/article01","title":{"rendered":"YONEDA Yoshihiro \u226aBiochemistry\u226b <span>\u201cA High-Resolution Structure of the Pre-microRNA Nuclear Export Machinery\u201d<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2009year\/article01\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><!-- \n\n<h1>\u7c73\u7530 \u60a6\u5553 \u226a\u751f\u5316\u5b66\u226b<\/h1>\n\n --><\/p>\n<p class=\"figure\"><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2009\/yoneda20091127.jpeg\" target=\"_blank\" class=\"zoom\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2009\/yoneda20091127.jpeg\" alt=\"\" height=\"223\" width=\"300\" \/><br \/>\n  <span class=\"click\">Click to enlarge<\/span><\/a><\/p>\n<p>2009-11-27<br \/>\n  <span class=\"lineFrame\">Publish<\/span> Science, 326, 1275-1279 (2009)<\/p>\n<p><!-- \n\n<h3>\u30de\u30a4\u30af\u30edRNA\u524d\u99c6\u4f53\u6838\u5916\u8f38\u9001\u8907\u5408\u4f53\u306eX\u7dda\u7d50\u6676\u69cb\u9020\u89e3\u6790<\/h3>\n\n\n\n\n<p>\u6838\u3067\u8ee2\u5199\u3055\u308c\u305f\u30de\u30a4\u30af\u30edRNA\u524d\u99c6\u4f53\u306e\u7d30\u80de\u8cea\u3078\u306e\u8f38\u9001\u306f\u3001\u30de\u30a4\u30af\u30edRNA\u306e\u751f\u5408\u6210\u306b\u304a\u3044\u3066\u5fc5\u9808\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u3042\u308a\u3001\u30a8\u30ad\u30b9\u30dd\u30fc\u30c6\u30a3\u30f35\uff08exportin-5\uff09\u3068\u547c\u3070\u308c\u308b\u8f38\u9001\u53d7\u5bb9\u4f53\u306b\u3088\u308a\u62c5\u308f\u308c\u3066\u3044\u308b\u3002\u672c\u8ad6\u6587\u3067\u306f\u3001\u30a8\u30ad\u30b9\u30dd\u30fc\u30c6\u30a3\u30f35\uff0d\u30e9\u30f3GTP\uff0d\u30de\u30a4\u30af\u30edRNA\u524d\u99c6\u4f53\u304b\u3089\u306a\u308b\u6838\u5916\u8f38\u9001\u8907\u5408\u4f53\u306e\u7d50\u6676\u69cb\u9020\u30922.9\u30aa\u30f3\u30b0\u30b9\u30c8\u30ed\u30fc\u30e0\u306e\u89e3\u50cf\u5ea6\u3067\u660e\u3089\u304b\u306b\u3057\u305f\u3002\u30a8\u30ad\u30b9\u30dd\u30fc\u30c6\u30a3\u30f35\uff0d\u30e9\u30f3GTP\u306f\u3001\u91ce\u7403\u306e\u30df\u30c3\u30c8\u69d8\u306e\u69cb\u9020\u3092\u3057\u3066\u304a\u308a\u3001\u30de\u30a4\u30af\u30edRNA\u524d\u99c6\u4f53\u306e2\u672c\u9396\u9818\u57df\u306e\u30ea\u30f3\u9178\u30d0\u30c3\u30af\u30dc\u30fc\u30f3\u3092\u7de9\u3084\u304b\u306b\u7d50\u5408\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u30df\u30c3\u30c8\u306e\u5e95\u90e8\u306b\u5f53\u305f\u308b\u90e8\u4f4d\u306b\u5b58\u5728\u3059\u308b\u30c8\u30f3\u30cd\u30eb\u72b6\u306e\u69cb\u9020\u306b\u3088\u308a\u30de\u30a4\u30af\u30edRNA\u524d\u99c6\u4f53\u306b\u7279\u6709\u306e3\u2019\u672b\u7aef\u7a81\u51fa\u69cb\u9020\u3092\u8a8d\u8b58\u3057\u3066\u3044\u305f\uff08\u56f3\uff09\u3002\u30a8\u30ad\u30b9\u30dd\u30fc\u30c6\u30a3\u30f3 5\uff0d\u30e9\u30f3GTP\u8907\u5408\u4f53\u306f\u3001\u5869\u57fa\u914d\u5217\u306b\u95a2\u308f\u308a\u306a\u304f\u30de\u30a4\u30af\u30edRNA\u524d\u99c6\u4f53\u3092\u8a8d\u8b58\u3067\u304d\u308b\u3002\u307e\u305f\u3001\u7279\u5fb4\u7684\u306a\u672b\u7aef\u69cb\u9020\u3092\u8b58\u5225\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u69d8\u3005\u306a\u30de\u30a4\u30af\u30edRNA\u524d\u99c6\u4f53\u3092\u7a4d\u307f\u8377\u3068\u3057\u3066\u8a8d\u8b58\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306b\u3088\u308b\u5206\u89e3\u304b\u3089\u4fdd\u8b77\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p>\n\n --><\/p>\n<p>Nuclear export of microRNAs (miRNAs) by exportin-5 (Exp-5) is an essential step in miRNA biogenesis. Here, we present the 2.9 angstrom structure of the pre-miRNA nuclear export machinery formed by pre-miRNA complexed with Exp-5 and a guanine triphosphate (GTP)-bound form of the small nuclear guanine triphosphatase (GTPase) Ran (RanGTP). The x-ray structure shows that Exp-5:RanGTP recognizes the 2-nucleotide 3&#8242; overhang structure and the double-stranded stem of the pre-miRNA. Exp-5:RanGTP shields the pre-miRNA stem from degradation in a baseball mitt-like structure where it is held by broadly distributed weak interactions, whereas a tunnel-like structure of Exp-5 interacts strongly with the 2-nucleotide 3&#8242; overhang through hydrogen bonds and ionic interactions. RNA recognition by Exp-5:RanGTP does not depend on RNA sequence, implying that Exp-5:RanGTP can recognize a variety of pre-miRNAs.<\/p>\n<p class=\"mt25\"><span class=\"lineFrame\">URL<\/span> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/pub\/anat3\/\" target=\"_blank\">http:\/\/www.med.osaka-u.ac.jp\/pub\/anat3\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Click to enlarge 2009-11-27 Publish Science, 326, 1275-1279 (2009) Nuclear export of microRNA [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1803,"parent":462,"menu_order":134,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/652"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=652"}],"version-history":[{"count":6,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/652\/revisions"}],"predecessor-version":[{"id":2371,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/652\/revisions\/2371"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/462"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/1803"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=652"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}