{"id":6539,"date":"2021-08-19T09:11:21","date_gmt":"2021-08-19T00:11:21","guid":{"rendered":"https:\/\/www.med.osaka-u.ac.jp\/eng\/?page_id=6539"},"modified":"2021-11-05T11:46:52","modified_gmt":"2021-11-05T02:46:52","slug":"tissue_biochemistry","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/introduction\/research\/biochemistry\/tissue_biochemistry","title":{"rendered":"Tissue Biochemistry"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n  <!-- \n\n<li>Website<\/li>\n\n --><\/p>\n<li><a href=\"https:\/\/www.med.osaka-u.ac.jp\/introduction\/research\/biochemistry\/tissue_biochemistry\">Text in Japanese<\/a><\/li>\n<p>  <!-- \n\n<li><a href=\"#\" target=\"_blank\" class=\"pdf\" rel=\"noopener\">\u5927\u5b66\u9662\u751f\u5411\u3051\u7814\u7a76\u6982\u8981<\/a><\/li>\n\n -->\n<\/ul>\n<div class=\"index\">\n<div class=\"title\">Understanding regulatory mechanisms for cell and tissue differentiation to study and treat diseases<\/div>\n<ul>\n<li>Understanding molecular mechanisms that regulate bone and cartilage formation<\/li>\n<li>Developing regenerative treatments for articular cartilage damage<\/li>\n<li>Discovering drugs that cure growth cartilage abnormalities<\/li>\n<\/ul>\n<\/div>\n<p><!-- \/ .index --><\/p>\n<div class=\"profile\">\n<div class=\"clearfix\">\n<div class=\"fl photo\">\n<p><img decoding=\"async\" class=\"\" src=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/11\/tissue_biochemistry_profile.png\" alt=\"\" \/><\/p>\n<\/p><\/div>\n<p>    <!-- \/ .photo --><\/p>\n<div class=\"name\"><span class=\"position\">Professor<\/span> Noriyuki Tsumaki<\/div>\n<p>    <!-- \/ .name --><\/p>\n<div class=\"member\">Tissue Biochemistry, <br \/>\n      Department of Biochemistry and Molecular Biology,<br \/>\n      Graduate School of Medicine<\/div>\n<p>    <!-- \/ .member --><\/p>\n<div class=\"description\">The skeleton is made of cartilage, which includes articular cartilage and growth cartilage. Abnormalities in articular cartilage cause joint dyskinesia (mobility impairment), while abnormalities in growth cartilage cause skeletal growth disorders.The treatment of these cartilage diseases is difficult and has been a challenging area of research for many years. In our laboratory, we are attempting to elucidate the pathogenesis of cartilage diseases by investigating the mechanisms that control cartilage formation and differentiation using biochemistry, molecular biology, and histology techniques.We are also working on the development of new cartilage regeneration therapies and drug discovery using iPS cell technology.<\/div>\n<p>    <!-- \/ .description --><\/div>\n<p>  <!-- \/ .clearfix --><\/div>\n<p><!-- \/ .profile --><\/p>\n<h3>We aim to understand the mechanisms of cell and tissue differentiation at the molecular level to control related pathologies and develop curative treatments<\/h3>\n<p class=\"figure\"><span class=\"caption\"><a href=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/bf67476988e6bc10c98c4671fdda0096.png\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-6611 \" src=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/bf67476988e6bc10c98c4671fdda0096-400x400.png?_t=1629333072\" alt=\"\" width=\"364\" height=\"364\" srcset=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/bf67476988e6bc10c98c4671fdda0096-400x400.png 400w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/bf67476988e6bc10c98c4671fdda0096-150x150.png 150w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/bf67476988e6bc10c98c4671fdda0096.png 480w\" sizes=\"(max-width: 364px) 100vw, 364px\" \/><\/a><br \/>\n  <\/span><\/p>\n<p>The skeleton and joints are indispensable for animals, including humans, to move their bodies. They are made from cartilage through a process called endochondral bone formation. Cartilage diseases thus impair skeletal formation and movements, but many have no curative treatments.Cartilage is tissue that consists of chondrocytes and cartilage extracellular matrix. Both are needed for cartilage to achieve its normal function. Our laboratory is investigating the mechanisms responsible for cartilage tissue formation by analyzing chondrocyte signaling and the function of matrix genes (J Cell Biol 1996; 1999; 2004; J Clin Invest 2011; Nature Commun 2016).<\/p>\n<p>As one approach, we employ genetically modified mice and analyze their phenotypes using biochemistry, molecular biology and histology techniques. Based on the findings obtained through such basic science research, we are developing curative treatments for cartilage diseases. <br \/>\n  We have also developed methods which induce iPS cells to differentiate into chondrocytes that produce cartilage extracellular matrix and ultimately grow into cartilage tissue (Nature 2014; Stem Cells Transl Med 2020). <br \/>\n  Transplantation experiments using this iPS cell-derived cartilage are being conducted as regenerative medicine for cartilage damage. For skeletal dysplasia, we are building cartilage models based on patient-specific iPS cells and conducting drug screening (Osteoarthritis Cartilage 2018; Sci Rep 2020).<\/p>\n<p class=\"figure\"><a href=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/6bcd15a3d4dc7ac9a0c12aa4a6924c29-1.jpg\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-6616 size-full\" src=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/6bcd15a3d4dc7ac9a0c12aa4a6924c29-1.jpg?_t=1629333626\" alt=\"\" width=\"959\" height=\"476\" srcset=\"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/6bcd15a3d4dc7ac9a0c12aa4a6924c29-1.jpg 959w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/6bcd15a3d4dc7ac9a0c12aa4a6924c29-1-400x199.jpg 400w, https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/2021\/08\/6bcd15a3d4dc7ac9a0c12aa4a6924c29-1-768x381.jpg 768w\" sizes=\"(max-width: 959px) 100vw, 959px\" \/><\/a><\/p>\n<p>We are analyzing the biological materials not as cells but as tissues consisting of cells and extracellular matrices to try and understand at the molecular level how tissues differentiate. Crucial to our approach is the combination of genetically modified mice, cell reprogramming, gene editing and single cell RNA-sequencing. By clarifying the pathologies at the molecular level, we aim to develop curative treatments for intractable diseases.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Understanding regulatory mechanisms for cell and tissue differentiation to study and treat di [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":70,"menu_order":169,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/6539"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=6539"}],"version-history":[{"count":16,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/6539\/revisions"}],"predecessor-version":[{"id":6864,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/6539\/revisions\/6864"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/70"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=6539"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}