{"id":655,"date":"2017-03-14T11:51:31","date_gmt":"2017-03-14T02:51:31","guid":{"rendered":"http:\/\/www2.med.osaka-u.ac.jp\/wwwmed\/eng\/activities\/results\/2009year\/nishida-yuya-tsujimoto-yoshihide"},"modified":"2017-04-28T15:31:38","modified_gmt":"2017-04-28T06:31:38","slug":"article04","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2009year\/article04","title":{"rendered":"NISHIDA Yuya, TSUJIMOTO Yoshihide \u226aMolecular Genetics\u226b <span>\u201cDiscovery of Atg5\/Atg7-independent alternative macroautophagy\u201d<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2009year\/article04\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><!-- \n\n<h1>\u897f\u7530 \u53cb\u54c9\u3001\u8fbb\u672c \u8cc0\u82f1 \u226a\u907a\u4f1d\u5b50\u5b66\u226b<\/h1>\n\n --><\/p>\n<p class=\"figure\"><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2009\/nishida20100108.jpeg\" target=\"_blank\" class=\"zoom\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2009\/nishida20100108.jpeg\" alt=\"\" height=\"250\" width=\"178\" \/><br \/>\n  <span class=\"click\">Click to enlarge<\/span><\/a><\/p>\n<p>2009-10-1<br \/>\n  <span class=\"lineFrame\">Publish<\/span> Nature 461: 654-659 (2009)<\/p>\n<p><!-- \n\n<h3>Atg5\/Atg7\u975e\u4f9d\u5b58\u7684\u306a\u65b0\u898f\u306e\u30de\u30af\u30ed\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u6a5f\u69cb\u306e\u767a\u898b<\/h3>\n\n\n\n\n<p>\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306f\u3001\u5fc5\u8981\u306b\u5fdc\u3058\u7d30\u80de\u5185\u5c0f\u5668\u5b98\u3092\u542b\u3080\u81ea\u5df1\u69cb\u6210\u6210\u5206\u3092\u5206\u89e3\u3059\u308b\u30b7\u30b9\u30c6\u30e0\u3067\u3001\u305d\u306e\u6a5f\u80fd\u7570\u5e38\u306f\u795e\u7d4c\u75be\u60a3\u3084\u767a\u764c\u306a\u3069\u69d8\u3005\u306a\u75be\u60a3\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u3002\u5f93\u6765\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306e\u5b9f\u884c\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u3001\u9032\u5316\u7684\u306b\u4fdd\u5b58\u3055\u308c\u3066\u3044\u308b\u8907\u6570\u306e\u5206\u5b50\u7fa4Atg5, Atg7, LC3\u7b49\u306b\u3088\u3063\u3066\u652f\u914d\u3055\u308c\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u304d\u305f\u304c\u3001\u79c1\u305f\u3061\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001Atg5, Atg7, LC3\u306a\u3069\u306e\u5206\u5b50\u306b\u4f9d\u5b58\u3057\u306a\u3044\u65b0\u305f\u306a\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u6a5f\u69cb\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u305f\u3002\u3053\u306e\u65b0\u898f\u30aa\u30fc\u30c8\u30d5\u30a1\u30b8\u30fc\u306f\u3001\u30b4\u30eb\u30b8\u88c5\u7f6e\u3084\u30a8\u30f3\u30c9\u30bd\u30fc\u30e0\u3092\u8d77\u6e90\u3068\u3057\u3066\u3044\u308b\u3002\u7d30\u80de\u306bDNA\u50b7\u5bb3\u306a\u3069\u306e\u30b9\u30c8\u30ec\u30b9\u304c\u52a0\u308f\u3063\u305f\u6642\u306b\u5f37\u304f\u8a98\u5c0e\u3055\u308c\u308b\u4ed6\u3001\u8d64\u8840\u7403\u304c\u6210\u719f\u3059\u308b\u969b\u306b\u8d77\u308b\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u9664\u53bb\u306b\u3082\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3002<\/p>\n\n --><\/p>\n<p>Macroautophagy is a process that leads to the bulk degradation of subcellular constituents by producing autophagosomes\/autolysosomes. It is believed that Atg5 (ref. 4) and Atg7 (ref. 5) are essential genes for mammalian macroautophagy. Here we show, however, that mouse cells lacking Atg5 or Atg7 can still form autophagosomes\/autolysosomes and perform autophagy-mediated protein degradation when subjected to certain stressors. Although lipidation of the microtubule-associated protein light chain 3 (LC3, also known as Map1lc3a) to form LC3-II is generally considered to be a good indicator of macroautophagy, it did not occur during the Atg5\/Atg7-independent alternative process of macroautophagy. We also found that this alternative process of macroautophagy was regulated by several autophagic proteins, including Unc-51-like kinase 1 (Ulk1) and beclin 1. Unlike conventional macroautophagy, autophagosomes seemed to be generated in a Rab9-dependent manner by the fusion of isolation membranes with vesicles derived from the trans-Golgi and late endosomes. In vivo, Atg5-independent alternative macroautophagy was detected in several embryonic tissues. It also had a function in clearing mitochondria during erythroid maturation. These results indicate that mammalian macroautophagy can occur through at least two different pathways: an Atg5\/Atg7-dependent conventional pathway and an Atg5\/Atg7-independent alternative pathway.<\/p>\n<p class=\"mt25\"><span class=\"lineFrame\">URL<\/span> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/pub\/gene\/www\/\" target=\"_blank\">http:\/\/www.med.osaka-u.ac.jp\/pub\/gene\/www\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Click to enlarge 2009-10-1 Publish Nature 461: 654-659 (2009) Macroautophagy is a process tha [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1806,"parent":462,"menu_order":171,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/655"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=655"}],"version-history":[{"count":6,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/655\/revisions"}],"predecessor-version":[{"id":2374,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/655\/revisions\/2374"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/462"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/1806"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=655"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}