{"id":674,"date":"2017-03-14T11:53:36","date_gmt":"2017-03-14T02:53:36","guid":{"rendered":"http:\/\/www2.med.osaka-u.ac.jp\/wwwmed\/eng\/activities\/results\/2011year\/murakami-masaakihirano-toshio"},"modified":"2017-04-28T16:08:22","modified_gmt":"2017-04-28T07:08:22","slug":"article07","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2011year\/article07","title":{"rendered":"MURAKAMI Masaaki,HIRANO Toshio \u226aDevelopmental Immunology\u226b <span>\u201cLocal microbleeding facilitates IL-6\u2013 and  IL-17\u2013dependent arthritis in the absence of tissue antigen recognition by  activated T cells\u201d<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2011year\/article07\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><!-- \n\n<h1> \u6751\u4e0a&#12288;\u6b63\u6643\u3001\u5e73\u91ce \u4fca\u592b\u226a\u514d\u75ab\u767a\u751f\u5b66\u226b<\/h1>\n\n --><\/p>\n<p class=\"figure\"><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2011\/murakami20110114.jpeg\" target=\"_blank\" class=\"zoom\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2011\/murakami20110114.jpeg\" alt=\"\u514d\u75ab\u767a\u751f\u5b66\" height=\"159\" width=\"219\" \/><br \/>\n  <span class=\"click\">Click to enlarge<\/span><\/a><\/p>\n<p>2011-1-10<br \/>\n  <span class=\"lineFrame\">Publish<\/span>J. Exp. Med.208 103-114(2011)<\/p>\n<p><!-- \n\n<h3>MHC class II\u4f9d\u5b58\u6027\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u306e\u767a\u75c7\u306e\u305f\u3081\u306e\uff14\u30b9\u30c6\u30c3\u30d7\u30e2\u30c7\u30eb<\/h3>\n\n\n\n\n<p>\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u3068\u306f\u3001\u672c\u6765\u81ea\u5206\u306e\u4f53\u3092\u75c5\u539f\u4f53\u304b\u3089\u5b88\u308b\u3079\u304d\u514d\u75ab\u7cfb\u304c\u3001\u81ea\u5206\u81ea\u8eab\u306e\u7d44\u7e54\u3001\u81d3\u5668\u3092\u653b\u6483\u3057\u3066\u201c\u96e3\u6cbb\u6027\u306e\u6162\u6027\u7684\u306a\u708e\u75c7\u201d\u3092\u8a98\u5c0e\u3059\u308b\u75c5\u6c17\u3067\u3042\u308b\uff0e\u591a\u304f\u306e\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u3067\u306f\u3001\u708e\u75c7\u304c\u8a98\u5c0e\u3055\u308c\u308b\u6a19\u7684\u81d3\u5668\u306f\u9650\u3089\u308c\u3066\u3044\u308b\u3002\u4f8b\u3048\u3070\u3001\u95a2\u7bc0\u30ea\u30a6\u30de\u30c1\u3067\u306f\u3001\u95a2\u7bc0\u3078\u306e\u708e\u75c7\u53cd\u5fdc\u304c\u305d\u306e\u75c5\u614b\u3092\u8a98\u5c0e\u3059\u308b\u3002\u3053\u308c\u307e\u3067\u3001\u5404\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u3054\u3068\u306e\u6a19\u7684\u81d3\u5668\u306e\u7279\u7570\u6027\u306e\u7406\u7531\u306f\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u8003\u3048\u3089\u308c\u3066\u304d\u305f\uff1a\u300e\u672c\u6765\u6b63\u5e38\u306a\u514d\u75ab\u7cfb\u304c\u6301\u3064\u81ea\u5df1\u6297\u539f\u306b\u5bfe\u3059\u308b\u30c8\u30ec\u30e9\u30f3\u30b9\u306e\u7834\u58ca\u304c\u539f\u56e0\u300f\u3002\u3053\u306e\u8003\u3048\u65b9\u306f\u3001\u307b\u3068\u3093\u3069\u306e\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u304c\u3001\u907a\u4f1d\u5b66\u7684\u306bCD4+T\u7d30\u80de\u304c\u6297\u539f\u3092\u8a8d\u8b58\u3059\u308b\u305f\u3081\u306b\u5fc5\u9808\u306a\u201cMHC\u30af\u30e9\u30b9\uff12\u201d\u907a\u4f1d\u5b50\u306b\u30ea\u30f3\u30af\u3059\u308b\u3053\u3068\u304b\u3089\u3082\u652f\u6301\u3055\u308c\u3066\u304d\u305f\u3002\u3057\u304b\u3057\u3001\u307b\u3068\u3093\u3069\u306e\u81ea\u5df1\u514d\u75ab\u75be\u60a3\u3067\u306f\u3001\u514d\u75ab\u7cfb\u304c\u653b\u6483\u3057\u3066\u3044\u308b\u201c\u81d3\u5668\u306e\u7279\u7570\u6297\u539f\u201d\u306f\u7279\u5b9a\u3055\u308c\u3066\u3044\u306a\u3044\u3002\u4eca\u56de\u3001\u306a\u305c\u3001\u3053\u306e\u3088\u3046\u306a\u7279\u7570\u6297\u539f\u304c\u5358\u96e2\u3067\u304d\u306a\u3044\u306e\u304b\u3092\u8aac\u660e\u3059\u308b\u30e2\u30c7\u30eb\u3092\u793a\u3057\u305f\u3002IL-6\u4f9d\u5b58\u6027\u306e\u30ea\u30a6\u30de\u30c1\u30e2\u30c7\u30eb\u3001F759\u30de\u30a6\u30b9\u306e\u95a2\u7bc0\u708e\u306fMHC\u30af\u30e9\u30b9\uff12\u3068CD4+T\u7d30\u80de\u306b\u4f9d\u5b58\u3059\u308b\u304c\u3001CD4+T\u7d30\u80de\u306e\u6297\u539f\u8a8d\u8b58\u306b\u306f\u4f9d\u5b58\u3057\u306a\u304b\u3063\u305f\u3002\u305d\u306e\u767a\u75c7\u6a5f\u5e8f\u3092\u691c\u8a0e\u3057\u305f\u6240\u3001\uff08\uff11\uff09CD4+T\u7d30\u80de\u306e\u6d3b\u6027\u5316\u306b\u4f34\u3046\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u767a\u73fe\u3001\uff08\uff12\uff09\u6d3b\u6027\u5316CD4+T\u7d30\u80de\u306e\u6a19\u7684\u81d3\u5668\u3078\u306e\u96c6\u7a4d\u3001\uff08\uff13\uff09\u6a19\u7684\u81d3\u5668\u3067\u306e\u4e00\u904e\u6027\u306eIL-6\u30a2\u30f3\u30d7\uff08\u9593\u8449\u7cfb\u7d30\u80de\u3067\u306eIL-17\u3068IL-6\u523a\u6fc0\u306b\u3088\u308bIL-6\u3084\u30b1\u30e2\u30ab\u30a4\u30f3\u7b49\u306e\u76f8\u4e57\u7684\u767a\u73fe\uff09\u306e\u6d3b\u6027\u5316\uff08\uff14\uff09\u6a19\u7684\u81d3\u5668\u306eT\u7d30\u80de\u7531\u6765\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u611f\u53d7\u6027\u306e\u4ea2\u9032\u306e\uff14\u3064\u306e\u30b9\u30c6\u30c3\u30d7\u304c\u91cd\u8981\u3067\u3042\u308b\u4e8b\u3092\u793a\u3057\u305f\u3002\u3053\u306e\uff14\u3064\u306e\u30b9\u30c6\u30c3\u30d7\u306e\u7d50\u679c\u3068\u3057\u3066\u3001 IL-6\u30a2\u30f3\u30d7\u306e\u6a19\u7684\u81d3\u5668\u306b\u304a\u3051\u308b\u201c\u6162\u6027\u7684\u201d\u6d3b\u6027\u5316\u4ea2\u9032\u304c\u8d77\u3053\u308a\u3001\u5927\u91cf\u306eIL-6\u3084\u30b1\u30e2\u30ab\u30a4\u30f3\u7b49\u304c\u5c40\u6240\u3067\u767a\u73fe\u3055\u308c\u3066\u81d3\u5668\u7279\u7570\u7684\u306a\u6162\u6027\u708e\u75c7\u306b\u767a\u5c55\u3059\u308b\u3002\u3055\u3089\u306b\u3001\u5b9f\u9a13\u7684\u8133\u810a\u9ac4\u708e\u306e\u5b9f\u9a13\u7d50\u679c\u3068\u5408\u308f\u305b\u3066\u8003\u3048\u308b\u3068\u201cCD4+T\u7d30\u80de\u306e\u6d3b\u6027\u5316\u201d\u306f\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u306e\u30bd\u30fc\u30b9\u3068\u3057\u3066\u91cd\u8981\u3067\u3042\u308b\u304c\u3001\u3053\u306e\u6d3b\u6027\u5316\u306f\u5fc5\u305a\u3057\u3082\u6a19\u7684\u81d3\u5668\u6297\u539f\u306b\u7279\u7570\u7684\u3067\u3042\u308b\u5fc5\u8981\u306f\u7121\u3044\u3002\u3055\u3089\u306b\u3001\u201c\u6d3b\u6027\u5316CD4+T\u7d30\u80de\u306e\u6a19\u7684\u81d3\u5668\u3078\u306e\u96c6\u7a4d\u201d\u306f\u3001\u5fc5\u305a\u3057\u3082\u6a19\u7684\u81d3\u5668\u6297\u539f\u306b\u4f9d\u5b58\u3057\u3066\u3044\u308b\u5fc5\u8981\u306f\u7121\u304f\u3001\u6a19\u7684\u81d3\u5668\u5c40\u6240\u3067\u306e\u611f\u67d3\u3001\u5916\u50b7\u3084\u7570\u7269\u3001\u3042\u308b\u3044\u306f\u5fae\u5c0f\u51fa\u8840\u306a\u3069\u306e\u201c\u5c40\u6240\u306e\u4e8b\u8c61\/ Local events\u201d\u306b\u3088\u3063\u3066\u8a98\u5c0e\u3055\u308c\u308b\u3002<\/p>\n\n --><\/p>\n<p>The recognition of specific antigens by CD4+ T cells is thought to play important roles in the development of autoimmune diseases, particularly when the diseases are associated with class II MHC alleles1,2. In many cases, however, the antigens that activate CD4+ T cells and directly induce the disease have not been identified, despite therapeutic strategies that target T cells attenuate disease symptoms3,4. Here we show that the development of class II MHC-associated autoimmune arthritis in F759 mice critically depends on the expression of IL-17A in CD4+ T cells, whereas specific antigen recognition by this T cell population does not appear to play a significant etiologic role. Our results demonstrate that the TCRV\u03b1 and TCRV\u03b2 profiles of CD4+ T cells in F759 mice with autoimmune arthritis are significantly perturbed. On the other hand, transgenic expression of a single version of the T cell receptor in RAG2-deficient F759 mice resulted in autoimmune arthritis accompanied by splenomegaly, lymphadenopathy, and increased Th17 cell numbers. Additionally, joint injections of Th17 cells, but not activated, nonpolarized CD4+ T cells precipitated arthritis by amplifying IL-6 signaling in type 1 collagen+ fibroblasts. Because forced expression of IL-17A in RAG2-deficient F759 mice induced the disease, these data demonstrate that IL-17A expression in CD4+ T cells rather than TCR recognition of specific antigens mediates the development of a class II MHC-associated autoimmune disease. Thus, some tissue-specific autoimmune disorders that are associated with class II MHC molecules may be caused by enhanced sensitivity to CD4+ T cell&ndash;derived cytokines in the affected tissues but not by antigen-recognitions of CD4+ T cells. These results may provide a foundation for new therapies for autoimmune diseases that are associated with class II MHC alleles but have not yet been linked to specific T cell antigens.<\/p>\n<p><span class=\"mt25\"><span class=\"lineFrame\">URL<\/span> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/pub\/molonc\/www\/\" target=\"_blank\">http:\/\/www.med.osaka-u.ac.jp\/pub\/molonc\/www\/<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Click to enlarge 2011-1-10 PublishJ. Exp. Med.208 103-114(2011) The recognition of specific a [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1797,"parent":460,"menu_order":183,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/674"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=674"}],"version-history":[{"count":7,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/674\/revisions"}],"predecessor-version":[{"id":2392,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/674\/revisions\/2392"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/460"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/1797"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=674"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}