{"id":676,"date":"2017-03-14T11:54:03","date_gmt":"2017-03-14T02:54:03","guid":{"rendered":"http:\/\/www2.med.osaka-u.ac.jp\/wwwmed\/eng\/activities\/results\/2012year\/kunimoto-koshitsukita-sachiko"},"modified":"2017-04-28T16:10:31","modified_gmt":"2017-04-28T07:10:31","slug":"article02","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2012year\/article02","title":{"rendered":"KUNIMOTO Koshi,TSUKITA Sachiko \u226aBiological Science\u226b <span>\u201cCoordinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet\u201d<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2012year\/article02\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><!-- \n\n<h1> \u56fd\u672c&#12288;\u6643\u53f8\u3001\u6708\u7530&#12288;\u65e9\u667a\u5b50\u226a\u5206\u5b50\u751f\u4f53\u60c5\u5831\u5b66\u226b<\/h1>\n\n --><\/p>\n<p class=\"figure\"><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2012\/kunimoto20120120.jpeg\" target=\"_blank\" class=\"zoom\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2012\/kunimoto20120120.jpeg\" alt=\"\u5206\u5b50\u751f\u4f53\u60c5\u5831\u5b66\" height=\"303\" width=\"247\" \/><br \/>\n  <span class=\"click\">Click to enlarge<\/span><\/a><\/p>\n<p>2012-1-20<br \/>\n  <span class=\"lineFrame\">Publish<\/span>Cell, 148, 189&ndash;200 (2012)<\/p>\n<p><!-- \n\n<h3>\u7e4a\u6bdb\u904b\u52d5\u306e\u65b9\u5411\u6027\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\uff5e\u6839\u3063\u3053\u304c\u304b\u306a\u3081\uff5e<\/h3>\n\n\n\n\n<p>\u30d2\u30c8\u306e\u6c17\u7ba1\u3084\u5375\u7ba1\u306e\u5185\u8154\u306f\u307e\u308b\u3067\u7d68\u6bef\u306e\u3088\u3046\u306b\u3073\u3063\u3057\u308a\u3068\u7e4a\u6bdb\u7d30\u80de\u306e\u30b7\u30fc\u30c8\u304c\u8986\u3063\u3066\u3044\u307e\u3059\u3002\u4e00\u3064\u306e\u7e4a\u6bdb\u7d30\u80de\u306f\u6bdb\u306e\u3088\u3046\u306a\u69cb\u9020\u3092100\u672c\u4ee5\u4e0a\u3082\u6301\u3063\u3066\u304a\u308a\u3001\u5168\u3066\u306e\u7d30\u80de\u306e\u5168\u3066\u306e\u7e4a\u6bdb\u304c\u307e\u308b\u3067\u30dc\u30fc\u30c8\u3092\u3053\u3050\u3088\u3046\u306b\u3001\u5171\u540c\u3057\u3066\u52d5\u304d\u3001\u6c17\u7ba1\u3067\u306f\u7d30\u83cc\u3084\u30a6\u30a4\u30eb\u30b9\u306a\u3069\u306e\u75c5\u539f\u4f53\u3001\u5375\u7ba1\u3067\u306f\u5375\u5b50\u3092\u904b\u3093\u3067\u3044\u307e\u3059\u3002\u4f55\u6545\u3001\u7e4a\u6bdb\u7d30\u80de\u304c\u3053\u306e\u3088\u3046\u306b\u3001\u898f\u5247\u6b63\u3057\u304f\u3001\u540c\u3058\u65b9\u5411\u306b\u52d5\u304f\u3053\u3068\u304c\u51fa\u6765\u308b\u306e\u304b\u306f\u8b0e\u3067\u3057\u305f\u304c\u3001\u6211\u3005\u306f\u3001\u30de\u30a6\u30b9\u306e\u907a\u4f1d\u5b50\u3092\u64cd\u4f5c\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u3053\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u89e3\u660e\u3059\u308b\u3053\u3068\u3092\u8a66\u307f\u307e\u3057\u305f\u3002\u7e4a\u6bdb\u306e\u6839\u5143\uff08\u57fa\u5e95\u5c0f\u4f53\uff09\u306b\u306f\u3001basal foot\u3068\u547c\u3070\u308c\u308b\u7279\u5fb4\u7684\u306a\u7a81\u8d77\u69cb\u9020\u304c\u3042\u308a\u307e\u3059\u3002\u6211\u3005\u306fOuter dense fiber 2 (Odf2)\u3068\u3044\u3046\u305d\u306e\u7a81\u8d77\u306b\u5b58\u5728\u3059\u308b\u86cb\u767d\u8cea\u3092\u5909\u7570\u3055\u305b\u305f\u30de\u30a6\u30b9\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001basal 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foot\u306f\u3001\u7d30\u80de\u5206\u88c2\u306b\u95a2\u308f\u308b\u4e2d\u5fc3\u4f53\u3068\u3044\u3046\u69cb\u9020\u306b\u3082\u4ed8\u7740\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u4e2d\u5fc3\u4f53\u3092\u6839\u5143\u306b\u3057\u3066\u7e4a\u6bdb\u304c\u751f\u3048\u308b\u3053\u3068\u3082\u308f\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u7a81\u8d77\u69cb\u9020\u3001\u7e4a\u6bdb\u3001\u4e2d\u5fc3\u4f53\u306e\u672c\u5f53\u306e\u5f79\u5272\u3092\u3055\u3089\u306b\u89e3\u660e\u3057\u3001\u591a\u7a2e\u591a\u69d8\u306a\u7e4a\u6bdb\u95a2\u9023\u306e\u75c5\u6c17\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u7406\u89e3\u3057\u3001\u6cbb\u7652\u3055\u305b\u308b\u3053\u3068\u304c\u51fa\u6765\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u6700\u7d42\u7684\u306b\u306f\u3001\u6c17\u7ba1\u306e\u7e4a\u6bdb\u7d30\u80de\u306e\u52d5\u304d\u3092\u8abf\u7bc0\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u6c17\u9053\u306e\u611f\u67d3\u75c7\u3084\u305d\u306e\u91cd\u75c7\u5316\u3092\u4e88\u9632\u3059\u308b\u3053\u3068\u304c\u3001\u51fa\u6765\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u5375\u7ba1\u304c\u539f\u56e0\u306e\u4e0d\u598a\u75c7\u306e\u65b9\u3082\u3001\u540c\u69d8\u306b\u6cbb\u7652\u3055\u305b\u308b\u3053\u3068\u304c\u51fa\u6765\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n --><\/p>\n<p>Coordinated beating of cilia in the trachea generates a directional flow of mucus required to clear the airways. Each cilium originates from a barrel-shaped basal body, from the side of which protrudes a structure known as the basal foot. We generated mice in which exons 6 and 7 of Odf2, encoding a basal body and centrosome-associated protein Odf2\/cenexin, are disrupted. Although Odf2\u2206Ex6,7\/\u2206Ex6,7 mice form cilia, ciliary beating is uncoordinated, and the mice display a coughing\/sneezing-like phenotype thus, considered as a new mouse model of primary ciliary diskinesia. While residual expression of the C-terminal region of Odf2 in these mice is sufficient for ciliogenesis, the resulting basal bodies lack basal feet. Loss of basal feet in ciliated epithelia disrupted the polarized organization of apical microtubule lattice, without affecting the asymmetrical localization of planar cell polarity protein Vangl1. The requirement for Odf2 in basal foot-formation therefore reveals a crucial role of this structure in the polarized alignment of basal bodies and coordinated ciliary beating.<\/p>\n<p><span class=\"mt25\"><span class=\"lineFrame\">URL<\/span><\/span><a href=\"http:\/\/www.fbs.osaka-u.ac.jp\/labs\/tsukita\/\">http:\/\/www.fbs.osaka-u.ac.jp\/labs\/tsukita\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Click to enlarge 2012-1-20 PublishCell, 148, 189&ndash;200 (2012) Coordinated beating of cili [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1787,"parent":459,"menu_order":166,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/676"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=676"}],"version-history":[{"count":6,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/676\/revisions"}],"predecessor-version":[{"id":2394,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/676\/revisions\/2394"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/459"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/1787"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=676"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}