{"id":686,"date":"2017-03-14T11:55:10","date_gmt":"2017-03-14T02:55:10","guid":{"rendered":"http:\/\/www2.med.osaka-u.ac.jp\/wwwmed\/eng\/activities\/results\/2014year\/nakada-shinichiro"},"modified":"2017-04-28T16:11:31","modified_gmt":"2017-04-28T07:11:31","slug":"article02","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2014year\/article02","title":{"rendered":"NAKADA Shinichiro \u226aBioregulation and Cellular Response\u226b <span>\u201cFine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice\u201d<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2014year\/article02\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><!-- \n\n<h1> \u4e2d\u7530 \u614e\u4e00\u90ce\u226a\u7d30\u80de\u5fdc\u7b54\u5236\u5fa1\u5b66\u226b<\/h1>\n\n --><\/p>\n<p class=\"figure\"><a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2014\/nakada20140220_s.jpeg\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2014\/nakada20140220_s.jpeg\" alt=\"\u514d\u75ab\u5236\u5fa1\u5b66-1\" height=\"385\" width=\"417\" \/><br \/>\n  <span class=\"click\">Click to enlarge<\/span><\/a> <\/p>\n<p>2014-2-20<br \/>\n  <span class=\"lineFrame\">Publish<\/span>Molecular Cell(2014) doi:10.1016\/j.molcel.2014.01.030\n<\/p>\n<p><!-- \n\n<h3>\u8131\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u9175\u7d20OTUB2\u306b\u3088\u308aDNA\u640d\u50b7\u5fdc\u7b54\u6027\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u304c\u9069\u5207\u306b\u5236\u5fa1\u3055\u308c\u3001DNA\u4fee\u5fa9\u65b9\u6cd5\u9078\u629e\u304c\u6b63\u3057\u304f\u884c\u3048\u308b\u74b0\u5883\u304c\u6574\u3048\u3089\u308c\u308b<\/h3>\n\n\n\n\n<p> \u653e\u5c04\u7dda\u7167\u5c04\u3084\u4e00\u90e8\u306e\u6297\u816b\u760d\u85ac\u306e\u52b9\u679c\u3001\u3055\u3089\u306b\u306f\u7d30\u80de\u306e\u4ee3\u8b1d\u306b\u3088\u308a\u3001\u7d30\u80de\u306b\u306fDNA2\u672c\u9396\u640d\u50b7\u304c\u767a\u751f\u3057\u307e\u3059\u3002DNA2\u672c\u9396\u640d\u50b7\u306f\u3001\u9069\u5207\u306b\u4fee\u5fa9\u3055\u308c\u306a\u3044\u3068\u7d30\u80de\u6b7b\u3084\u907a\u4f1d\u5b50\u5909\u7570\u3092\u5f15\u304d\u8d77\u3053\u3059\u975e\u5e38\u306b\u5371\u967a\u306aDNA\u640d\u50b7\u3067\u3059\u3002DNA2\u672c\u9396\u640d\u50b7\u306e\u4fee\u5fa9\u306b\u304a\u3044\u3066\u306f\u3001\u300c\u76f8\u540c\u7d44\u63db\u3048\u4fee\u5fa9\u300d\u3068\u300c\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\u300d\u3068\u3044\u30462\u3064\u65b9\u6cd5\u304c\u3042\u308a\u3001\u72b6\u6cc1\u306b\u5fdc\u3058\u3066\u4f7f\u3044\u5206\u3051\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u4fee\u5fa9\u65b9\u6cd5\u9078\u629e\u306e\u5206\u5b50\u6a5f\u69cb\u306f\u3001\u73fe\u5728\u6ce8\u76ee\u3092\u96c6\u3081\u3066\u3044\u308b\u7814\u7a76\u5bfe\u8c61\u3067\u3059\u3002\u4eca\u56de\u3001\u6211\u3005\u306f\u3001\u8131\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u9175\u7d20\u3067\u3042\u308bOTUB2\u304c\u3001DNA2\u672c\u9396\u640d\u50b7\u90e8\u4f4d\u3067\u304a\u3053\u308b\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u6291\u5236\u7684\u306b\u5236\u5fa1\u3057\u3001DNA\u4fee\u5fa9\u65b9\u6cd5\u306e\u9078\u629e\u304c\u9069\u5207\u306b\u884c\u3048\u308b\u74b0\u5883\u3092\u6574\u5099\u3057\u3066\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002OTUB2\u306e\u767a\u73fe\u304c\u6291\u5236\u3055\u308c\u305f\u7d30\u80de\u3067\u306f\u3001\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u4f9d\u5b58\u6027\u306b\u76f8\u540c\u7d44\u63db\u3048\u4fee\u5fa9\u6291\u5236\u5206\u5b5053BP1\u30fbRAP80\u304c\u904e\u5270\u306b\u96c6\u7a4d\u3059\u308b\u305f\u3081\u3001\u76f8\u540c\u7d44\u63db\u3048\u4fee\u5fa9\u304c\u9078\u629e\u3055\u308c\u306b\u304f\u304f\u3001\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\u304c\u9078\u629e\u3055\u308c\u3084\u3059\u3044\u74b0\u5883\u3068\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002OTUB2\u306f\u904e\u5270\u306a\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u3067\u300153BP1\u30fbRAP80\u306e\u6025\u6fc0\u306a\u96c6\u7a4d\u3092\u6291\u5236\u3057\u3001\u76f8\u540c\u7d44\u63db\u3048\u4fee\u5fa9\u306e\u9078\u629e\u3092\u53ef\u80fd\u306b\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u3001DNA2\u672c\u9396\u640d\u50b7\u90e8\u4f4d\u306b\u8d77\u3053\u308b\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u3068DNA\u4fee\u5fa9\u3068\u306e\u95a2\u9023\u306f\u307b\u3068\u3093\u3069\u308f\u304b\u3063\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001\u672c\u7814\u7a76\u306b\u3088\u308a\u305d\u306e\u4e00\u7aef\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002<br \/>\n<\/p>\n\n --><\/p>\n<p> DNA double-strand breaks (DSBs) are deleterious lesions that lead to genetic mutations and cell death. Most of DSBs are repaired by homologous recombination or non-homologous end joining. Accumulating evidences suggest that DNA repair pathway choice is an important mechanism for maintaining genomic stability. In this study, we revealed that the deubiquitinating enzyme OTUB2 fine-tunes the speed of DSB-induced ubiquitination so that the appropriate DNA repair pathway is chosen. Depletion of OTUB2 enhances DSB-induced ubiquitination and accelerates accumulation of 53BP1 and RAP80 at DSBs, which in turn inhibits homologous recombination and promotes non-homologous end joining. OTUB2 suppress excessive ubiquitination and enables homologous recombination.<\/p>\n<p><span class=\"lineFrame\">URL<\/span><a href=\"http:\/\/www.bcr.med.osaka-u.ac.jp\/public_html\/index.html\">http:\/\/www.bcr.med.osaka-u.ac.jp\/public_html\/index.html<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese Click to enlarge 2014-2-20 PublishMolecular Cell(2014) doi:10.1016\/j.molcel.2014.01.030 DNA d [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1780,"parent":457,"menu_order":166,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/686"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=686"}],"version-history":[{"count":6,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/686\/revisions"}],"predecessor-version":[{"id":2404,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/686\/revisions\/2404"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/457"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/1780"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}