{"id":710,"date":"2017-03-14T11:56:57","date_gmt":"2017-03-14T02:56:57","guid":{"rendered":"http:\/\/www2.med.osaka-u.ac.jp\/wwwmed\/eng\/activities\/results\/2016year\/miyishi-eiji"},"modified":"2017-04-28T16:13:51","modified_gmt":"2017-04-28T07:13:51","slug":"article20","status":"publish","type":"page","link":"https:\/\/www.med.osaka-u.ac.jp\/eng\/activities\/results\/2016year\/article20","title":{"rendered":"MIYISHI Eiji \u226aMolecular Biochemistry and Clinical Investigation\u226b <span>\u201cCore Fucosylation on T cells, Required for Activation of T-cell Receptor Signaling and Induction of Colitis in Mice, is Increased in Patients with Inflammatory Bowel Disease\u201d<\/span>"},"content":{"rendered":"<ul class=\"linkBar clearfix\">\n<li> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/activities\/results\/2016year\/article20\">Text in Japanese<\/a><\/li>\n<\/ul>\n<p><!-- \n\n<h1> MIYOSHI Eiji &lt;Molecular Biochemistry and Clinical Investigation&gt;<\/h1>\n\n --><\/p>\n<p>2016-03-07<br \/>\n  <span class=\"lineFrame\">Publish<\/span>Gastroenterology\uff082016\uff09 doi:10.1053\/j.gastro.2016.03.002<\/p>\n<p class=\"figure\"> <a href=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2016\/miyoshi20160307.jpeg\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.med.osaka-u.ac.jp\/eng\/wp-content\/uploads\/\/activities\/results\/2016\/miyoshi20160307.jpeg\" alt=\"\" height=\"281\" width=\"379\" \/><br \/>\n  <span class=\"click\">Click to enlarge<\/span><\/a><\/p>\n<p><!-- \n\n<h3> Core Fucosylation on T cells, Required for Activation of T-cell Receptor Signaling and Induction of Colitis in Mice, is Increased in Patients with Inflammatory Bowel Disease<\/h3>\n\n --><\/p>\n<p>Core fucosylation is one of the most important glycosylation involved in cancer and inflammation. The synthesis of core fucose is catalyzed by \u03b11-6 fucosyltransferase (FUT8). In the present study, we investigated whether core fucosylation regulates T-cell activation and induction of colitis in mice, and is altered in patients with inflammatory bowel disease (IBD). Levels of core fucosylation were increased on T cells in both animal colitis models and IBD patients. Fut8\uff0d\/\uff0d mice developed less-severe colitis than Fut8+\/+ mice due to decreases in T cell signaling. Adoptive transfer of Fut8\uff0d\/\uff0d T cells to RAG2-deficient mice also reduced the severity of colitis. TCR complexes formed on CD4+ T cells from Fut8\uff0d\/\uff0d mice did not signal properly following activation and were not transported to lipid rafts as a result from electron microscopy. Taken together, core fucosylation of the TCR is required for T-cell signaling and production of inflammatory cytokines, and induction of colitis in mice. Levels of TCR core fucosylation are increased on T cells from intestinal tissues of patients with IBD; this process might be blocked as a therapeutic strategy.\n<\/p>\n<p><span class=\"lineFrame\">URL<\/span><a href=\"http:\/\/sahswww.med.osaka-u.ac.jp\/~tousa\/index.html\" target=\"_blank\">http:\/\/sahswww.med.osaka-u.ac.jp\/~tousa\/index.html<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Text in Japanese 2016-03-07 PublishGastroenterology\uff082016\uff09 doi:10.1053\/j.gastro.2016.03.002 Click to enlarge Co [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1702,"parent":455,"menu_order":203,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/710"}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/comments?post=710"}],"version-history":[{"count":7,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/710\/revisions"}],"predecessor-version":[{"id":2420,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/710\/revisions\/2420"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/pages\/455"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media\/1702"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/eng\/wp-json\/wp\/v2\/media?parent=710"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}