Michio Asahi, M.D, Ph.D.

Assistant Professor

Department of Biochemistry

Osaka University Graduate School of Medicine

Room B1, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

Phone: 81-6-6879-3421

Fax: 81-6-6879-3429

E-mail: m.asahi@biochem.med.osaka-u.ac.jp

Education:

1987 M.D., Osaka Med. Col.

1996 Ph.D., Osaka Univ. Grad. Sch. of Med.

1987-1990 Medical training (General Internal Medicine and Cardiology), Osaka National Hospital

1990-1992 Medical training (Cardiology), Aijinkai Takatsuki Hostital

and Yao Municipal Hospital

1996-1997 Dept. of Medicine and Therapeutics, Osaka Univ. Grad. Sch. of Med.

1998-2002 Post-doctoral fellow, Banting and Best Medical Research, Univ. of Toronto

2002-2003 Clinical fellow, Dept. of Medicine and Therapeutics, Osaka Univ. Grad. Sch. of Med.

2003-2004 Post-doctoral fellow, Dept. of Biochemistry, Osaka Univ. Grad. Sch. of Med.

2005- Assistant Professor, Dept. of Biochemistry, Osaka Univ. Grad. Sch. of Med.

Recent Publication:

1. Asahi M, Fujii J, Suzuki K, Seo HG, Kuzuya T, Hori M, Tada M, Fujii S, Taniguchi N. Inactivation of glutathione peroxidase by nitric oxide. Implication for cytotoxicity.

J Biol Chem. 1995;270:21035-9.

2. Asahi M, Fujii J, Takao T, Kuzuya T, Hori M, Shimonishi Y, Taniguchi N.

The oxidation of selenocysteine is involved in the inactivation of glutathione peroxidase by nitric oxide donor.

J Biol Chem. 1997;272:19152-7.

3. Che W, Asahi M, Takahashi M, Kaneto H, Okado A, Higashiyama S, Taniguchi N. Selective induction of heparin-binding epidermal growth factor-like growth factor by methylglyoxal and 3-deoxyglucosone in rat aortic smooth muscle cells. The involvement of reactive oxygen species formation and a possible implication for atherogenesis in diabetes.

J Biol Chem. 1997;272:18453-9.

4. Kimura Y, Asahi M, Kurzydlowski K, Tada M, MacLennan DH.

Phospholamban domain Ib mutations influence functional interactions with the Ca2+-ATPase isoform of cardiac sarcoplasmic reticulum.

J Biol Chem. 1998;273:14238-41.

5.　 Asahi M, Kimura Y, Kurzydlowski K, Tada M, MacLennan DH.

Transmembrane helix M6 in sarco(endo)plasmic reticulum Ca(2+)-ATPase forms a functional interaction site with phospholamban. Evidence for physical interactions at other sites.

J Biol Chem. 1999;274:32855-62.

6. Yamashita N, Hoshida S, Otsu K, Asahi M, Kuzuya T, Hori M.

Exercise provides direct biphasic cardioprotection via manganese superoxide dismutase activation.

J Exp Med. 1999;189:1699-706.

7. Asahi M, McKenna E, Kurzydlowski K, Tada M, MacLennan DH.

Physical interactions between phospholamban and sarco(endo)plasmic reticulum Ca2+-ATPases are dissociated by elevated Ca2+, but not by phospholamban phosphorylation, vanadate, or thapsigargin, and are enhanced by ATP.

J Biol Chem. 2000;275:15034-8.

8. Asahi M, Green NM, Kurzydlowski K, Tada M, MacLennan DH.

Phospholamban domain IB forms an interaction site with the loop between transmembrane helices M6 and M7 of sarco(endo)plasmic reticulum Ca2+ ATPases. Proc Natl Acad Sci U S A. 2001;98:10061-6.

9. Asahi M, Kurzydlowski K, Tada M, MacLennan DH.

Sarcolipin inhibits polymerization of phospholamban to induce superinhibition of sarco(endo)plasmic reticulum Ca2+-ATPases (SERCAs).

J Biol Chem. 2002;277:26725-8.

10. Tupling AR*, Asahi M*, MacLennan DH. *Contributed equally.

Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs contractile function.

J Biol Chem. 2002;277:44740-6.

11. Asahi M, Sugita Y, Kurzydlowski K, De Leon S, Tada M, Toyoshima C,

MacLennan DH.

Sarcolipin regulates sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) by binding to transmembrane helices alone or in association with phospholamban.

Proc Natl Acad Sci U S A. 2003;100:5040-5.

12. Haghighi K, Kolokathis F, Pater L, Lynch RA, Asahi M, Gramolini AO, Fan GC,

Tsiapras D, Hahn HS, Adamopoulos S, Liggett SB, Dorn GW, 2nd, MacLennan DH, Kremastinos DT, Kranias EG.

Human phospholamban null results in lethal dilated cardiomyopathy revealing a critical difference between mouse and human.

J Clin Invest. 2003;111:869-76.

13. Schmitt JP*, Kamisago M*, Asahi M*, Li GH, Ahmad F, Mende U, Kranias EG,

MacLennan DH, Seidman JG, Seidman CE. *Contributed equally.

Dilated cardiomyopathy and heart failure caused by a mutation in phospholamban. Science. 2003;299:1410-3.

14. Toyoshima C, Asahi M, Sugita Y, Khanna R, Tsuda T, MacLennan DH.

Modeling of the inhibitory interaction of phospholamban with the Ca2+ ATPase.

Proc Natl Acad Sci U S A. 2003;100:467-72.

15. Yamaguchi O, Higuchi Y, Hirotani S, Kashiwase K, Nakayama H, Hikoso S, Takeda T,

Watanabe T, Asahi M, Taniike M, Matsumura Y, Tsujimoto I, Hongo K, Kusakari Y, Kurihara S, Nishida K, Ichijo H, Hori M, Otsu K.

Targeted deletion of apoptosis signal-regulating kinase 1 attenuates left ventricular remodeling.

Proc Natl Acad Sci U S A. 2003;100:15883-8.

16. Asahi M, Otsu K, Nakayama H, Hikoso S, Takeda T, Gramolini AO, Trivieri MG,

Oudit GY, Morita T, Kusakari Y, Hirano S, Hongo K, Hirotani S, Yamaguchi O,

Peterson A, Backx PH, Kurihara S, Hori M, MacLennan DH.

Cardiac-specific overexpression of sarcolipin inhibits sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA2a) activity and impairs cardiac function in mice.

Proc Natl Acad Sci U S A. 2004;101:9199-204.

17. Tupling AR, Gramolini AO, Duhamel TA, Kondo H, Asahi M, Tsuchiya SC,

Borrelli MJ, Lepock JR, Otsu K, Hori M, MacLennan DH, Green HJ. HSP70 binds to the fast-twitch skeletal muscle Sarco (endo)plasmic reticulum Ca2+-ATPase (SERCA1a) and prevents thermal inactivation.

J Biol Chem. 2004;279:52382-52389.

18. Yamaguchi O, Watanabe T, Nishida K, Kashiwase K, Higuchi Y, Takeda T, Hikoso S,

Hirotani S, Asahi M, Taniike M, Nakai A, Tsujimoto I, Matsumura Y, Miyazaki J, Chien KR, Matsuzawa A, Sadamitsu C, Ichijo H, Baccarini M, Hori M, Otsu K. Cardiac-specific disruption of the c-raf-1 gene induces cardiac dysfunction and apoptosis.

J Clin Invest. 2004;114:937-43.

19. Hikoso S, Yamaguchi O, Higuchi Y, Hirotani S, Takeda T, Kashiwase K, Watanabe T,

Taniike M, Tsujimoto I, Asahi M, Matsumura Y, Nishida K, Nakajima H, Akira S,

Hori M, Otsu K.

Pressure overload induces cardiac dysfunction and dilation in signal transducer and activator of transcription 6-deficient mice.

Circulation 2004;110:2631-2637.

20. Gramolini AO, Kislinger T, Asahi M, Li W, Emili A, MacLennan DH.

Sarcolipin retention in the endoplasmic reticulum depends on its C-terminal RSYQY sequence and its interaction with sarco(endo)plasmic Ca(2+)-ATPases.

Proc Natl Acad Sci U S A. 2004;101:16807-16812.