{"id":1466,"date":"2021-10-06T10:30:24","date_gmt":"2021-10-06T01:30:24","guid":{"rendered":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/?p=1466"},"modified":"2021-10-06T14:26:03","modified_gmt":"2021-10-06T05:26:03","slug":"single-molecule-rna-sequencing-for-simultaneous-detection-of-m6a-and-5mc-scientific-reports-2021","status":"publish","type":"post","link":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/archives\/1466","title":{"rendered":"Single-Molecule RNA Sequencing for Simultaneous Detection of m6A and 5mC (Scientific Reports, 2021)"},"content":{"rendered":"<p><strong>Single-Molecule RNA Sequencing\u00a0for Simultaneous Detection of m6A and 5mC\u00a0(Scientific Reports, 2021)<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p>Epitranscriptomics is the study of RNA base modifications involving functionally relevant changes to the transcriptome. We have proposed a single-molecule quantum sequencer for mapping RNA base modifications in microRNAs (miRNAs), such as N6-methyladenosine (m6A)\u00a0or 5-methylcytidine (5mC). Here, we investigated 5mC and m6A in cancer cells; the rates were comparable to those determined by mass spectrometry. The methylation ratio of cytidine and adenosine is facilitated by the presence of vicinal methylation. Our work provides a robust new tool for sequencing various types of RNA base modifications.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Single-Molecule RNA Sequencing\u00a0for Simultaneous Detection of m6A and 5mC\u00a0(Scientific Reports, 2021) &nbsp; Epi &hellip; <a href=\"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/archives\/1466\" class=\"more-link\"><span class=\"screen-reader-text\">&#8220;Single-Molecule RNA Sequencing for Simultaneous Detection of m6A and 5mC (Scientific Reports, 2021)&#8221; \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3],"tags":[],"class_list":["post-1466","post","type-post","status-publish","format-standard","hentry","category-results"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/posts\/1466","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/comments?post=1466"}],"version-history":[{"count":1,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/posts\/1466\/revisions"}],"predecessor-version":[{"id":1467,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/posts\/1466\/revisions\/1467"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/media?parent=1466"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/categories?post=1466"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/mds\/en\/wp-json\/wp\/v2\/tags?post=1466"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}