{"id":65,"date":"2026-02-21T17:49:17","date_gmt":"2026-02-21T17:49:17","guid":{"rendered":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/?p=65"},"modified":"2026-02-23T14:53:48","modified_gmt":"2026-02-23T14:53:48","slug":"%e8%b6%85%e8%87%a8%e7%95%8c%e6%b5%81%e4%bd%93","status":"publish","type":"post","link":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/%e8%b6%85%e8%87%a8%e7%95%8c%e6%b5%81%e4%bd%93\/","title":{"rendered":"\u8d85\u81e8\u754c\u6d41\u4f53"},"content":{"rendered":"\n
\"\"<\/figure>\n\n\n\n

<\/p>\n\n\n\n

\u8d85\u81e8\u754c\u6d41\u4f53 (supercritical fluid: SCF) \u306f\u3001\u6e29\u5ea6\u3068\u5727\u529b\u304c\u6c17\u6db2\u306e\u81e8\u754c\u70b9 (critical point) \u3092\u8d85\u3048\u305f\u72b6\u614b\u306e\u6d41\u4f53\u3067\u3042\u308a\u3001\u6db2\u4f53\u306e\u6301\u3064\u6eb6\u89e3\u6027\u3068\u6c17\u4f53\u306e\u6301\u3064\u62e1\u6563\u6027\u306e\u4e21\u65b9\u306e\u7269\u6027\u3092\u6301\u3061\u5408\u308f\u305b\u305f\u7406\u60f3\u306e\u6eb6\u5a92\u3068\u3044\u3048\u307e\u3059\u3002\u6e29\u5ea6\u3068\u5727\u529b\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067\uff0c\u6eb6\u5a92\u7269\u6027\u3092\u81ea\u7531\u306b\u5909\u5316\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3053\u3068\u304b\u3089\u3001\u7528\u9014\u306b\u5fdc\u3058\u305f\u6eb6\u5a92\u6027\u80fd\u3092\u4ed8\u4e0e\u3067\u304d\u308b\u9ad8\u6a5f\u80fd\u306e\u5a92\u4f53\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u8d85\u81e8\u754c\u6d41\u4f53\u3092\u5229\u7528\u3057\u305f\u6280\u8853\u306f\u3001\u6709\u6a5f\u6eb6\u5a92\u306e\u4f7f\u7528\u91cf\u3092\u8efd\u6e1b\u3067\u304d\u308b\u3053\u3068\u304b\u3089\u3001\u74b0\u5883\u8ca0\u8377\u3084\u30b3\u30b9\u30c8\u306e\u9762\u3067\u6709\u5229\u306a\u6280\u8853\u3067\u3059\u3002\u8d85\u81e8\u754c\u6d41\u4f53\u306f\u3053\u308c\u307e\u3067\u306b\u62bd\u51fa\u3001\u5206\u96e2\u30fb\u7cbe\u88fd\u3001\u53cd\u5fdc\u306a\u3069\u306b\u304a\u3044\u3066\u5229\u7528\u3055\u308c\u3066\u304a\u308a\u3001\u305d\u306e\u4ed6\u306b\u3082\u6d17\u6d44\u3001\u67d3\u8272\u3001\u7c89\u672b\u5316\u3001\u767a\u6ce1\u306a\u3069\u5e45\u5e83\u3044\u5206\u91ce\u3067\u8d85\u81e8\u754c\u6d41\u4f53\u306e\u7279\u5fb4\u3092\u751f\u304b\u3057\u305f\u5229\u7528\u65b9\u6cd5\u304c\u691c\u8a0e\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

SCF\u306e\u4e2d\u3067\u3082\u8d85\u81e8\u754c\u4e8c\u9178\u5316\u70ad\u7d20 (supercritical carbon dioxide: SC\u2012CO2) \u306f\u3001(1) \u81e8\u754c\u6e29\u5ea6 (31.1 \u00b0C)\u3001\u81e8\u754c\u5727\u529b (7.38 MPa) \u304c\u6bd4\u8f03\u7684\u4f4e\u3044\u3001(2) \u591a\u304f\u306e\u6709\u6a5f\u5316\u5408\u7269\u3092\u6eb6\u89e3\u3059\u308b\u3001(3) \u5316\u5b66\u7684\u306b\u4e0d\u6d3b\u6027\u3001(4) \u7121\u6bd2\u3067\u5b89\u4fa1\u3001\u3068\u3044\u3063\u305f\u6027\u8cea\u304b\u3089\u6700\u3082\u3088\u304f\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u99ac\u5834\u7814\u7a76\u5ba4\u3067\u306f\u3001\u30e1\u30bf\u30dc\u30ed\u30fc\u30e0\u89e3\u6790\u306b\u304a\u3051\u308b\u300c\u62bd\u51fa\u300d\u304a\u3088\u3073\u300c\u5206\u96e2\u300d\u30d7\u30ed\u30bb\u30b9\u306bSC\u2012CO2\u306e\u6d3b\u7528\u3092\u8a66\u307f\u3066\u304d\u307e\u3057\u305f1)<\/sup>\u3002\u8d85\u81e8\u754c\u6d41\u4f53\u3092\u62bd\u51fa\u5a92\u4f53\u3068\u3057\u3066\u4f7f\u7528\u3057\u305f\u62bd\u51fa\u64cd\u4f5c\u306f\u8d85\u81e8\u754c\u6d41\u4f53\u62bd\u51fa (supercritical fluid extraction: SFE) \u3068\u547c\u3070\u308c\u3001\u307e\u305f\u3001\u8d85\u81e8\u754c\u6d41\u4f53\u3092\u79fb\u52d5\u76f8\u3068\u3057\u3066\u4f7f\u7528\u3057\u305f\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306f\u8d85\u81e8\u754c\u6d41\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc (supercritical fluid chromatography: SFC) \u3068\u547c\u3070\u308c\u3066\u3044\u307e\u3059\u3002\u3055\u3089\u306b\u3001SFE\u3068SFC\u3092\u30aa\u30f3\u30e9\u30a4\u30f3\u3067\u63a5\u7d9a\u3057\u305f\u8d85\u81e8\u754c\u6d41\u4f53\u62bd\u51fa\uff0d\u8d85\u81e8\u754c\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc (supercritical fluid extraction\u2012supercritical fluid chromatography: SFE\u2012SFC) \u306f\u62bd\u51fa\u3068\u5206\u6790\u3092\u5358\u4e00\u884c\u7a0b\u3067\u884c\u3046\u3053\u3068\u3092\u53ef\u80fd\u3068\u3057\u305f\u624b\u6cd5\u3067\u3059\u3002<\/p>\n\n\n\n

SC\u2012CO2\u306e\u6975\u6027\u306f\u30d8\u30ad\u30b5\u30f3\u7a0b\u5ea6\u3068\u3055\u308c\u308b\u305f\u3081\u3001\u758e\u6c34\u6027\u5316\u5408\u7269\u306e\u5206\u96e2\u30fb\u5206\u6790\u306b\u6709\u7528\u3067\u3042\u308b\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u5f53\u521d\u306f\u3001\u79fb\u52d5\u76f8\u306b100%\u306e\u4e8c\u9178\u5316\u70ad\u7d20\u3092\u4f7f\u7528\u3057\u3066\u3001\u6e29\u5ea6\u3068\u5727\u529b\u306e\u5024\u3092\u5909\u66f4\u3057\u305f\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u4e3b\u6d41\u3067\u3057\u305f\u304c\u3001\u6700\u8fd1\u3067\u306f\u3001SC\u2012CO2\u306b\u5bfe\u3057\u3066\u30e1\u30bf\u30ce\u30fc\u30eb\u306a\u3069\u306e\u6709\u6a5f\u6eb6\u5a92\u3092\u30e2\u30c7\u30a3\u30d5\u30a1\u30a4\u30a2\u3068\u3057\u3066\u6dfb\u52a0\u3057\u305f\u4e9c\u81e8\u754c\u6d41\u4f53\u3068\u3057\u3066\u5229\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u591a\u304f\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u30e2\u30c7\u30a3\u30d5\u30a1\u30a4\u30a2\u3092\u6dfb\u52a0\u3059\u308b\u3053\u3068\u3067\u3001\u6975\u6027\u5316\u5408\u7269\u306e\u79fb\u52d5\u76f8\u306b\u5bfe\u3059\u308b\u6eb6\u89e3\u529b\u3092\u9ad8\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u5e45\u5e83\u3044\u7269\u6027\u3092\u6709\u3059\u308b\u5316\u5408\u7269\u985e\u3092\u540c\u6642\u306b\u5206\u96e2\u30fb\u5206\u6790\u3067\u304d\u308b\u304b\u3089\u3067\u3059\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u8d85\u81e8\u754c\u6d41\u4f53\u6280\u8853\u306e\u5b9f\u7528\u4f8b\u3068\u3057\u3066\u3001\u6211\u3005\u306fSFC\/MS\u306b\u3088\u308b\u8102\u8cea\u5206\u5b50\u306e\u4e00\u6589\u5206\u6790 (\u30ea\u30d4\u30c9\u30fc\u30e0\u89e3\u6790) \u306e\u6280\u8853\u958b\u767a\u3092\u884c\u3063\u3066\u304d\u307e\u3057\u305f\u3002SFC\u306b\u7cbe\u5bc6\u8cea\u91cf\u3092\u53d6\u5f97\u3059\u308b\u3068\u3068\u3082\u306bMS2\u6e2c\u5b9a\u304c\u5b9f\u65bd\u53ef\u80fd\u306a\u56db\u91cd\u6975\u30aa\u30fc\u30d3\u30c8\u30e9\u30c3\u30d7\u578b\u8cea\u91cf\u5206\u6790\u8a08\u3092\u63a5\u7d9a\u3059\u308b\u3053\u3068\u3067\u3001\u30de\u30a6\u30b9\u8840\u6f3f\u62bd\u51fa\u7269\u304b\u3089500\u7a2e\u4ee5\u4e0a\u306e\u8102\u8cea\u5206\u5b50\u7a2e\u306e\u540c\u5b9a\u3001\u5b9a\u91cf\u306b\u6210\u529f\u3057\u3066\u3044\u307e\u30592)<\/sup>\u3002\u307e\u305f\u3001SFE\u306f\u76ee\u7684\u6210\u5206\u304c\u542b\u307e\u308c\u308b\u5bfe\u8c61\u7269\u306b\u62bd\u51fa\u5a92\u4f53\u306eSCF\u3092\u52a0\u3048\u3001\u6eb6\u89e3\u5ea6\u306e\u5dee\u3092\u5229\u7528\u3057\u3066\u62bd\u51fa\u64cd\u4f5c\u3092\u884c\u3046\u624b\u6cd5\u3067\u3059\u3002\u4e00\u822c\u7684\u306a\u6709\u6a5f\u6eb6\u5a92\u62bd\u51fa\u6cd5\u3068\u6bd4\u3079\u3066\u3001\u9ad8\u3044\u62bd\u51fa\u52b9\u7387\u3001\u6697\u9ed2\u3001\u7121\u9178\u7d20\u4e0b\u3067\u306e\u30de\u30a4\u30eb\u30c9\u306a\u6761\u4ef6\u3067\u306e\u62bd\u51fa\u304c\u53ef\u80fd\u3067\u3042\u308a\u4f4e\u74b0\u5883\u8ca0\u8377\u306a\u3069\u306e\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u6211\u3005\u306f\u3053\u306e\u3088\u3046\u306aSFE\u306e\u7279\u6027\u3068SFC\/MS\u306e\u5229\u70b9\u3092\u6700\u5927\u9650\u306b\u751f\u304b\u3057\u305f\u30aa\u30f3\u30e9\u30a4\u30f3SFE-SFC\/MS\u306b\u3088\u308b\u4ee3\u8b1d\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u624b\u6cd5\u306b\u3064\u3044\u3066\u3082\u691c\u8a0e\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u30aa\u30f3\u30e9\u30a4\u30f3SFE-SFC\/MS\u306f\u65b0\u751f\u5150\u30de\u30b9\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3084\u907a\u4f1d\u6027\u75be\u60a3\u306e\u8a3a\u65ad\u3001\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u5b9f\u65bd\u3059\u308b\u305f\u3081\u306e\u308d\u7d19\u8840\u8a66\u6599\u306b\u9069\u7528\u53ef\u80fd\u306a\u6280\u8853\u3067\u3059\u3002\u5fae\u91cf\u306e\u30de\u30a6\u30b9\u8840\u6f3f (3 \u03bcL) \u304b\u3089\u8abf\u88fd\u3057\u305f\u308d\u7d19\u8840\u3092\u7528\u3044\u3066\u30aa\u30f3\u30e9\u30a4\u30f3SFE-SFC\/MS\u306b\u3088\u308b\u30ea\u30f3\u8102\u8cea\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u3092\u5b9f\u65bd\u3057\u305f\u3068\u3053\u308d134\u7a2e\u985e\u306e\u30ea\u30f3\u8102\u8cea\u306e\u5b9a\u91cf\u306b\u3082\u6210\u529f\u3057\u3066\u3044\u307e\u30593)<\/sup>\u3002<\/p>\n\n\n\n

\u3053\u306e\u3088\u3046\u306b\u3001\u8d85\u81e8\u754c\u6d41\u4f53\u6280\u8853\u306f\u5316\u5408\u7269\u306e\u300c\u62bd\u51fa\u300d\u3068\u300c\u5206\u96e2\u300d\u306b\u304a\u3051\u308b\u6eb6\u5a92\u306e\u5229\u7528\u53ef\u80fd\u6027\u3092\u5e83\u3052\u308b\u65b0\u6280\u8853\u3067\u3059\u3002\u5f90\u3005\u306b\u305d\u306e\u6709\u7528\u6027\u306f\u5e83\u307e\u308a\u3064\u3064\u3042\u308a\u307e\u3059\u304c\u3001\u4eca\u5f8c\u3082\u8d85\u81e8\u754c\u6d41\u4f53\u306e\u7279\u6027\u3092\u5229\u7528\u3057\u305f\u65b0\u3057\u3044\u5206\u6790\u6cd5\u306e\u958b\u767a\u3084\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3092\u958b\u767a\u3057\u3066\u3044\u304d\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u5206\u6790\u6280\u8853\u306e\u958b\u767a\u3067\u306f\u5b9a\u91cf\u30ea\u30d4\u30c9\u30df\u30af\u30b9\u306e\u65b0\u5206\u6790\u6cd5\u306b\u3064\u3044\u3066\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u662f\u975e\u3054\u4e00\u8aad\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\u53c2\u8003\u6587\u732e<\/strong><\/p>\n\n\n\n

    \n
  1. Bamba T, Lee JW, Matsubara A et al : Metabolic profiling of lipids by supercritical fluid chromatography\/mass spectrometry. J Chromatogr A 1250, 212\u2013219 (2012).<\/li>\n\n\n\n
  2. Yamada T, Uchikata T, Sakamoto S et al : Supercritical fluid chromatography\/orbitrap mass spectrometry based lipidomics platform coupled with automated lipid identification software for accurate lipid profiling. J. Chromatogr. A 1301, 237\u2013242 (2013).<\/li>\n\n\n\n
  3. Uchikata T, Matsubara A, Fukusaki E et al : High-throughput phospholipid profiling system based on supercritical fluid extraction-supercritical fluid chromatography\/mass spectrometry for dried plasma spot analysis. J. Chromatogr. A 1250, 69\u201375 (2012).<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    \u8d85\u81e8\u754c\u6d41\u4f53 (supercritical fluid: SCF) \u306f\u3001\u6e29\u5ea6\u3068\u5727\u529b\u304c\u6c17\u6db2\u306e\u81e8\u754c\u70b9 (critical point) \u3092\u8d85\u3048\u305f\u72b6\u614b\u306e\u6d41\u4f53\u3067\u3042\u308a\u3001\u6db2\u4f53\u306e\u6301\u3064\u6eb6\u89e3\u6027\u3068\u6c17\u4f53\u306e\u6301\u3064\u62e1\u6563\u6027\u306e\u4e21\u65b9\u306e\u7269\u6027\u3092\u6301\u3061\u5408\u308f\u305b\u305f\u7406\u60f3 […]<\/p>\n","protected":false},"author":1,"featured_media":66,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"vkexunit_cta_each_option":"","footnotes":""},"categories":[14,12],"tags":[],"class_list":["post-65","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-introduction","category-research"],"_links":{"self":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/posts\/65","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/comments?post=65"}],"version-history":[{"count":1,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/posts\/65\/revisions"}],"predecessor-version":[{"id":68,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/posts\/65\/revisions\/68"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/media\/66"}],"wp:attachment":[{"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/media?parent=65"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/categories?post=65"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.med.osaka-u.ac.jp\/pub\/sysmet\/wp-json\/wp\/v2\/tags?post=65"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}