TAKEDA Junji ≪Environmental and Molecular Medicine≫ “Genome-wide phenotype analysis in ES cells by regulated disruption of the Bloom’s syndrome gene.”
2004-6-24
Publish Nature 429, 896-899, 2004
The limitation of phenotype-based genetic screening in mammalian systems is the diploid nature of the genome. We used a tetracycline-regulated Bloom’s syndrome gene to introduce bi-allelic mutations across the genome in mouse ES cells.
