- Study of mechanisms and consequences of genomic rearrangement mutations in mammalian cells.
- Exploration of genomic architecture for long-range cis-regulation.
- Uncovering the roles of Wnt/Lrp6 signaling in development and disease.
- Etiology and treatment for pediatric neurometabolic disorders.
Recent Publications:
Kokubu C., The revolution that CRISPR/Cas has brought in genome-wide genetic screening. Journal of Clinical and Experimental Medicine (Igaku no Ayumi) 252(2): 177-181, 2015. (in Japanese)
Tokunaga M, Kokubu C, Maeda Y, Sese J, Horie K, Sugimoto N, Kinoshita T, Yusa K, and Takeda J. Simulation and estimation of gene number in a biological pathway using almost complete saturation mutagenesis screening of haploid mouse cells. BMC Genomics. 15(1): 1016, 2014
[ Pubmed ]
Igawa K., Kokubu C., Yusa K., Horie K., Yoshimura Y., Yamauchi K., Suemori H., Yokozeki H., Toyoda M., Kiyokawa N., Okita H., Miyagawa Y., Akutsu H., Umezawa A., Katayama I. and Takeda J. Removal of reprogramming transgenes improves tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells. STEM CELLS Translational Medicine. 3(9): 992-1001. 2014
[ Pubmed ]
Kokubu C. and Takeda J. When half is better than the whole: advances in haploid embryonic stem cell technology. Cell Stem Cell. 14(3): 265-7. 2014.
[ Pubmed ]
Takimoto A, Mohri H, Kokubu C, Hiraki Y, Shukunami C. Pax1 acts as a negative regulator of chondrocyte maturation. Exp Cell Res. 319: 3128-3139. 2013
[ Pubmed ]
Yamanishi A., Yusa K., Horie K., Tokunaga M., Kusano K., Kokubu C*. and Takeda J., Enhancement of microhomology-mediated genomic rearrangements by transient loss of mouse Bloom syndrome helicase. Genome Research. 23(9):1462-73. 2013 (*Co-corresponding author)
[ Pubmed ]
Horie K, Kokubu C, Yoshida J, Akagi K, Isotani A, Oshitani A, Yusa K, Ikeda R, Huang Y, Bradley A, Takeda J. A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening. Nat Methods, 8: 1071-1077, 2011.
[ Pubmed ]
Horie K, Kokubu C, Takeda J. Functional genomics in the mouse using the sleeping beauty transposon system. Methods Enzymol 477: 71-89. 2010.
[ Pubmed ]
Castelo-Branco G, Andersson ER, Minina E, Sousa KM, Ribeiro D, Kokubu C, Imai K, Prakash N, Wurst W, Arenas E. Delayed dopaminergic neuron differentiation in Lrp6 mutant mice. Dev Dyn. (2010); 239: 211-21.
[ Pubmed ]
*Kokubu C., Horie K., Abe K., Ikeda R., Mizuno S., Uno Y., Ogiwara S., Ohtsuka M., Isotani A., Okabe M., Imai K. and Takeda J. A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice. Nature Genetics (2009); 41: 946-952. (* Co-corresponding author)
[ Pubmed ]
Kubota T, Michigami T, Sakaguchi N, Kokubu C, Suzuki A, Namba N, Sakai N. Nakajima S, Imai K, Ozono K. Lrp6 hypomorphic mutation affects bone mass through bone resorption in mice and impairs interaction with Mesd. J Bone Miner Res. (2008); 23:1661-1671.
[ Pubmed ]
Kawai M, Mushiake S, Bessho K, Murakami M, Namba N, Kokubu C, Michigami T, Ozono K. Wnt/Lrp/-catenin signaling suppresses adipogenesis by inhibiting mutual activation of PPAR and C/EBP. Biochem Biophys Res Commun. (2007); 363: 276-282.
[ Pubmed ]
Ikeda R, Kokubu C, Yusa K, Keng VW, Horie K, Takeda J. Sleeping beauty transposase has an affinity for heterochromatin conformation. Mol Cell Biol. (2007); 27:1665-76.
[ Pubmed ]
Keng VW, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jankins NA, Copeland NG, Horie K, and Takeda J. Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system. Nature Methods (2005); 2: 763-769.
[ Pubmed ]
Kokubu C, Heinzmann U, Kokubu T, Sakai N, Kubota T, Kawai M, Wahl MB, Galceran J, Grosschedl R, Ozono K and Imai K. Skeletal defects in ringelschwanz mutant mice reveal that Lrp6 is required for proper somitogenesis and osteogenesis. Development (2004); 131: 5469-5480.
[ Pubmed ]
Kokubu C, Wilm B, Kokubu T, Wahl M, Rodrigo I, Sakai N, Santagati F, Hayashizaki Y, Suzuki M, Yamamura K, Abe K, Imai K. Undulated short-tail deletion mutation in the mouse ablates Pax1 and leads to ectopic activation of neighboring Nkx2-2 in domains that normally express Pax1. Genetics. (2003);165: 299-307.
[ Pubmed ]
Muramatsu T, Sakai N, Yanagihara L, Yamada M, Nishigaki T, Kokubu C, Tsukamoto H, Ito M, Inui K. Mutation analysis of the acid ceramidase gene in Japanese patients with Farber disease. J Inherit Metab Dis. (2002); 25: 585-92.
[ Pubmed ]
Santagati F, Gerber JK, Blusch JH, Kokubu C, Peters H, Adamski J, Werner T, Balling R, Imai K. Comparative analysis of the genomic organization of Pax9 and its conserved physical association with Nkx2-9 in the human, mouse, and pufferfish genomes. Mamm Genome. (2001); 12: 232-7.
[ Pubmed ]
Akagi M, Inui K, Nakajima S, Shima M, Nishigaki T, Muramatsu T, Kokubu C, Tsukamoto H, Sakai N, Okada S. Mutation analysis of two Japanese patients with Fanconi-Bickel syndrome. J Hum Genet. (2000); 45: 60-2.
[ Pubmed ]
Akagi M, Inui K, Nishigaki T, Muramatsu T, Kokubu C, Fu L, Fukushima H, Yanagihara I, Tsukamoto H, Kurahashi H, Okada S. Mutation analysis of a Japanese patient with fucosidosis. J Hum Genet. (1999); 44: 323-6.
[ Pubmed ]
Fu L, Inui K, Nishigaki T, Tatsumi N, Tsukamoto H, Kokubu C, Muramatsu T, Okada S. Molecular heterogeniety of Krabbe disease. J Inherit Metab Dis. (1999); 22: 155-62
[ Pubmed ]
Osugi Y, Hara J, Kokubu C, Okuda Y, Hosoi G, Okamura T, Imanaka H, Taenaka N, Tawa A, Okada S. Treatment of fulminant hemophagocytic lymphohistiocytosis with combined administration of OKT-3 and etoposide. International Journal of Pediatric Hematology/Oncology (1997); 4: 433-439.
Nanao S, Tanaka Y, Sakurai Y, Kokubu C, Watanabe Y, Masuda M and Yasui I. Pair distribution function in icosahedral Al-Mn-Si alloy. Mater. Sci. Eng. (1988); 99: 427-30
Sakurai Y, Kokubu C, Tanaka Y, Watanabe Y, Masuda M and Nanao S. The structure of icosahedral aluminum-magnesium-copper alloy. Mater. Sci. Eng. (1988); 99: 423-26
Doyama M, Yamamoto R, Kaneko T, Imafuku M, Kokubu C, Izumiya T and Hanamure T. The first observation of RHEED intensity oscillation during the growth of a metal-metal multilayered film by MBE and the electrical resistivity measurement of Mo/Al multilayered films grown by rf sputtering. Vacuum (1986); 36: 909-911.
Kaneko T, Imafuku M, Kokubu C, Yamamoto R, Doyama M. The first observation of RHEED intensity oscillation during the growth of Cu/Mo multi-layered films. Journal of the Physical Society of Japan (1986); 55: 2903-2904.
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