• Investigation into differentiation-regulating mechanisms in murine ES cells
• Searching for a new factor involved in differentiation of hematopoietic cells
• Effect of leukemogenic tyrosine kinases on the lineage-specific cell growth

Recent Publications:

Tokunaga M, Kokubu C, Maeda Y, Sese J, Horie K, Sugimoto N, Kinoshita T, Yusa K, and Takeda J. Simulation and estimation of gene number in a biological pathway using almost complete saturation mutagenesis screening of haploid mouse cells. BMC Genomics. 15(1): 1016, 2014
[ Pubmed ]

Yamanishi A., Yusa K., Horie K., Tokunaga M., Kusano K., Kokubu C. and Takeda J., Enhancement of microhomology-mediated genomic rearrangements by transient loss of mouse Bloom syndrome helicase. Genome Research. 23(9):1462-73. 2013
[ Pubmed ]

Matsui K., Ezoe S., Oritani K., Shibata M., Tokunaga M., Fujita N., Tanimura A., Sudo T., Tanaka H., McBurney M. W., Matsumura I. and Kanakura Y. NAD-dependent histone deacetylase, SIRT1, plays essential roles in the maintenance of hematopoietic stem cells. Biochem Biophys Res Commun. (2012); 418: 811-817.

Shibata M., Ezoe S., Oritani K., Matsui K., Tokunaga M., Fujita N., Saito Y., Takahashi T., Hino M., Matsumura I. and Kanakura Y. Predictability of the response to tyrosine kinase inhibitors via in vitro analysis of Bcr-Abl phosphorylation. Leuk Res. (2011); 35: 1205-1211.

Fujita J., Mizuki M., Otsuka M., Ezoe S., Tanaka H., Satoh Y., Fukushima K., Tokunaga M., Matsumura I. and Kanakura Y. Myeloid neoplasm-related gene abnormalities differentially affect dendritic cell differentiation from murine hematopoietic stem/progenitor cells. Immunol Lett. (2011); 136: 61-73.

Tokunaga M., Ezoe S., Tanaka H., Satoh Y., Fukushima K., Matsui K., Shibata M., Tanimura A., Oritani K., Matsumura I. and Kanakura Y. BCR-ABL but not JAK2 V617F inhibits erythropoiesis through the Ras signal by inducing p21^CIP1/WAF1. J Biol Chem. (2010); 285: 31774-31782.

Fukushima K., Matsumura I., Ezoe S., Tokunaga M., Yasumi M., Satoh Y., Shibayama H., Tanaka H., Iwama A. and Kanakura Y. FIP1L1-PDGFRalpha imposes eosinophil lineage commitment on hematopoietic stem/progenitor cells. J Biol Chem. (2009); 284: 7719-7732.

Satoh Y., Matsumura I., Tanaka H., Ezoe S., Fukushima K., Tokunaga M., Yasumi M., Shibayama H., Mizuki M., Era T., Okuda T. and Kanakura Y. AML1/RUNX1 works as a negative regulator of c-Mpl in hematopoietic stem cells. J Biol Chem. (2008); 283: 30045-30056.

Tokunaga M., Miyamura K., Ohashi H., Ishiwada N., Terakura S., Ikeguchi M., Kuwatsuka Y., Inamoto Y., Oba T., Tsuchiya S. and Kodera Y. Successful nonmyeloablative bone marrow transplantation for leukocyte adhesion deficiency type I from an unrelated donor. Int J Hematol. (2007); 86: 91-95.

Inamoto Y., Oba T., Miyamura K., Terakura S., Tsujimura A., Kuwatsuka Y., Tokunaga M., Kasai M., Murata M., Naoe T. and Kodera Y. Stable engraftment after a conditioning regimen with fludarabine and melphalan for bone marrow transplantation from an unrelated donor. Int J Hematol. (2006); 83: 356-362.

Masumoto N., Sasaki T., Tahara M., Mammoto A., Ikebuchi Y., Tasaka K., Tokunaga M., Takai Y. and Miyake A. Involvement of Rabphilin-3A in cortical granule exocytosis in mouse eggs. J Cell Biol. (1996); 135: 1741-1747.