- Establishment of new tools for forward genetic screen in human iPS cells
- Development of human disease models using the bi-allelic mutated mouse ES cell bank
Somatic cells derived from Bloom (BLM) syndrome patients show an increased rate of homologous recombination. This phenotype is caused by the lack of BLM helicase activity, resulting in generation of bi-allelic mutation from mono-allelic mutation. We previously established a tetracycline-regulated Blm allele to introduce bi-allelic mutations in mouse ES cells. Now we are applying this system to human iPS cells. We believe that the establishment of this method will open a novel avenue for understanding a variety of human diseases.
Recent Publications:
Igawa K., Kokubu C., Yusa K., Horie K., Yoshimura Y., Yamauchi K., Suemori H., Yokozeki H., Toyoda M., Kiyokawa N., Okita H., Miyagawa Y., Akutsu H., Umezawa A., Katayama I. and Takeda J. Removal of reprogramming transgenes improves tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells. STEM CELLS Translational Medicine. 3(9): 992-1001. 2014
[ Pubmed ]
Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.
Oda Y, Yoshimura Y, Ohnishi H, Tadokoro M, Katsube Y, Sasao M, Kubo Y, Hattori K, Saito S, Horimoto K, Yuba S, Ohgushi H.
J Biol Chem. 2010 Sep 17;285(38):29270-8.
Nested genomic structure of haploid germ cell specific haspin gene.
Yoshimura Y, Tanaka H, Nozaki M, Yomogida K, Yasunaga T, Nishimune Y.
Gene. Apr 4;267(1):49-54.(2001)
Identification and characterization of a haploid germ cell-specific nuclear protein
kinase (Haspin) in spermatid nuclei and its effects on somatic cells.
Tanaka H, Yoshimura Y, Nozaki M, Yomogida K, Tsuchida J, Tosaka Y, Habu T, Nakanishi T, Okada M, Nojima H, Nishimune Y.
J Biol Chem. Jun 11;274(24):17049-57.(1999)
Targeted disruption of the Rad51 gene leads to lethality in embryonic mice
T.Tsuzuki, Y.Fujii, K.Sakumi, Y.Tominaga, K.Nakao, M.Sekiguchi,A.Matushiro, Y.Yoshimura and T.Morita
Proc.Natl.Acad.Sci.USA 93:6236-6240.(1996)
A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes.
Morita T, Yoshimura Y, Yamamoto A, Murata K, Mori M, Yamamoto H, Matsushiro A.
Proc Natl Acad Sci U S A. Jul 15;90(14):6577-80.(1993)
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