Drosophila male accessory gland (AG) is a non-regenerative tissue and acquires cell death-resistances for long-living (unpublished). We found that AG cells were sometimes eliminated with aging, meaning that malfunctioning cells produced by aging are limitedly eliminated. In this research project, I revealed the tissue responses during detection and elimination of malfunctioning cells in AG as following; 1) decreasing of Paired protein level, 2) activation of Notch･JAK-STAT signals correlated with decreasing of Paired level, 3) activation of Pvr signal, and 4) elimination of cells mediated by apoptosis, autophagy and phagocytosis. These results imply that Notch･JAK-STAT･Pvr signals are involved in the detection and elimination of malfunctioning cell. As a result of further genetic analyses, we revealed that Pvr signal, which was induced by abnormality of cells, triggered the induction of cell death. In contrast, Notch･JAK-STAT signals, which were induced by reduction of cell function, permitted the Pvr signal-inducible cell death. We hypothesized that these two independent responses enable AG to detect and eliminate highly malfunctioning cells (unpublished).