RESULTS

Single-Molecule RNA Sequencing for Simultaneous Detection of m6A and 5mC (Scientific Reports, 2021)

Epitranscriptomics is the study of RNA base modifications involving functionally relevant changes to the transcriptome. We have proposed a single-molecule quantum sequencer for mapping RNA base modifications in microRNAs (miRNAs), such as N6-methyladenosine (m6A) or 5-methylcytidine (5mC). Here, we investigated 5mC and m6A in cancer cells; the rates were comparable to those determined by mass spectrometry. The methylation ratio of cytidine and adenosine is facilitated by the presence of vicinal methylation. Our work provides a robust new tool for sequencing various types of RNA base modifications.